Human ARF1 knockout HeLa cell lysate
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ARF1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 1 bp insertion in exon 2.
View Alternative Names
ADP-ribosylation factor 1, ARF1_HUMAN
- WB
Lab
Western blot - Human ARF1 knockout HeLa cell lysate (AB257353)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : ARF1 knockout HeLa cell lysate 20 μg
Lane 3 : MDA-MB-231 cell lysate 20 μg
Lane 4 : PANC-1 cell lysate 20 μg
Lanes 1 - 4 : Merged signal (red and green). Green - ab183576 observed at 18 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.
ab183576 was shown to react with ARF1 in wild-type HeLa cells in Western blot with loss of signal observed in ARF1 knockout cell line ab264939 (ARF1 knockout cell lysate ab257353). Wild-type HeLa and ARF1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab183576 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-ARF1 antibody (<a href='/en-us/products/primary-antibodies/arf1-antibody-ab183576'>ab183576</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ARF1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ARF1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-arf1-knockout-hela-cell-line-ab264939'>ab264939</a>)
Lane 3:
MDA-MB-231 cell lysate at 20 µg
Lane 4:
PANC-1 cell lysate at 20 µg
Predicted band size: 21 kDa
Observed band size: 18 kDa
false
- WB
Lab
Western blot - Human ARF1 knockout HeLa cell lysate (AB257353)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : ARF1 knockout HeLa cell lysate 20 μg
Lanes 1 - 2 : Merged signal (red and green). Green - ab58578 observed at 18 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55 kDa.
ab58578 was shown to react with ARF1 in wild-type HeLa cells in Western blot with loss of signal observed in ARF1 knockout cell line ab264939 (ARF1 knockout cell lysate ab257353). Wild-type HeLa and ARF1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab58578 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4 °C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Donkey anti-Goat IgG H&L (IRDye® 800CW) preabsorbed (ab216775) and Donkey anti-Mouse 680RD secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
All lanes:
Western blot - Anti-ARF1 antibody (<a href='/en-us/products/primary-antibodies/arf1-antibody-ab58578'>ab58578</a>) at 1 µg/mL
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ARF1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ARF1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-arf1-knockout-hela-cell-line-ab264939'>ab264939</a>)
Predicted band size: 21 kDa
Observed band size: 18 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ARF1 knockout HeLa cell lysate (AB257353)
Allele-2 : 1 bp insertion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human ARF1 knockout HeLa cell lysate (AB257353)
Allele-1 : 1 bp deletion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ARF1 is essential in regulating membrane dynamics and vesicular traffic. It forms part of the COPI and clathrin-coated vesicle complexes where it recruits coat proteins to budding vesicles. This recruitment is fundamental for maintaining Golgi structure and function. Additionally ARF1 plays a role in cytokinesis by interacting with the septin cytoskeleton. The protein also influences actin cytoskeleton remodeling which is pivotal for cell shape changes and motility.
Pathways
ARF1 is central to both the endocytic and secretory pathways. It collaborates with proteins like ARFS and 3F1 in modulating the trafficking of cargo between the endoplasmic reticulum and Golgi. Within the secretory pathway ARF1 interacts with SNARE proteins to facilitate vesicle docking and fusion. Its actions in pathways maintain cellular homeostasis and promote proper cellular response to various stimuli.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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