Human ARF5 knockout HEK-293T cell lysate
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ARF5 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 13 bp deletion in exon1 and 1 bp insertion in exon1.
View Alternative Names
ADP-ribosylation factor 5, ARF5_HUMAN
- Sanger seq
Unknown
Sanger Sequencing - Human ARF5 knockout HEK-293T cell lysate (AB257840)
Allele-2 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human ARF5 knockout HEK-293T cell lysate (AB257840)
Allele-1 : 13 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ARF5 is involved in modulating key cellular processes including membrane trafficking and lipid metabolism. It directly participates in the formation of vesicle coats by aiding the recruitment of coat protein complexes. ARF5 also interacts within cellular complexes impacting the transport of cargo in the secretory pathway. It also affects actin cytoskeleton remodeling thereby influencing cell shape and motility.
Pathways
The ARF5 protein contributes to both the COPI and clathrin-coated vesicle formation pathways. It functions within intricate signaling networks coordinating with other small GTPases such as ARF1. ARF5's activity links to pivotal roles within these pathways affecting protein and lipid sorting. These pathways are vital for maintaining cellular homeostasis and facilitating effective intracellular transport.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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