Human ARFIP1 (Arfaptin-1) knockout HEK-293T cell lysate
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ARFIP1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon7 and 8 bp deletion in exon7.
View Alternative Names
ADP-ribosylation factor-interacting protein 1, ARFIP 1, ARFP1_HUMAN, Arfaptin, Arfaptin-1, HSU52521, MGC117369
- Sanger seq
Unknown
Sanger Sequencing - Human ARFIP1 (Arfaptin-1) knockout HEK-293T cell lysate (AB257841)
Allele-2 : 8 bp deletion in exon7
- Sanger seq
Unknown
Sanger Sequencing - Human ARFIP1 (Arfaptin-1) knockout HEK-293T cell lysate (AB257841)
Allele-1 : 14 bp deletion in exon7
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Arfaptin-1 plays a part in intracellular transport and membrane receptor regulation. It is also recognized as a component of larger protein complexes contributing to the reorganization of actin cytoskeleton. Through its interactions with GTPases Arfaptin-1 influences endocytosis and exocytosis affecting cell surface receptor turnover. This adaptability allows it to play important roles in cellular communication and response to environmental stimuli.
Pathways
Arfaptin-1 is integral in cellular trafficking and signal transduction pathways. It is involved in the ARF small GTPase pathway influencing vesicle formation and trafficking. Arfaptin-1 also interacts with other proteins like Rac1 in the regulation of actin dynamics impacting the actin cytoskeleton remodeling pathway. These interactions highlight its central position in coordinating cellular architecture and material transport.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB251081
Anti-Arfaptin-1 antibody [EPR16360] - BSA and Azide free
RabMAb|Recombinant
![Western blot - Anti-Arfaptin-1 antibody [EPR16360] - BSA and Azide free (AB251081)](https://content.abcam.com/products/images/arfaptin-1-antibody-epr16360-bsa-and-azide-free-ab251081--western-blot-img46467.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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