JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB257356

Human ARHGDIA (RhoGDI) knockout HEK-293T cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

ARHGDIA KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.

View Alternative Names

ARHGDIA, GDIA 1, GDIR1_HUMAN, MGC117248, NPHS8, Rho GDI, Rho GDI 1, Rho GDP dissociation inhibitor (GDI) alpha, Rho GDP dissociation inhibitor alpha, Rho GDP-dissociation inhibitor 1, Rho-GDI alpha, RhoGDI 1, RhoGDI1, fa96g11, wu:fa96g11, zgc:55554, zgc:77681

4 Images
Western blot - Human ARHGDIA (RhoGDI) knockout HEK-293T cell lysate (AB257356)
  • WB

Lab

Western blot - Human ARHGDIA (RhoGDI) knockout HEK-293T cell lysate (AB257356)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : ARHGDIA knockout HEK293T cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)

ab108977 was shown to specifically react with RhoGDI in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266447 (knockout cell lysate ab257356) was used. Wild-type and RhoGDI knockout samples were subjected to SDS-PAGE. ab108977 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-RhoGDI antibody [EPR3772] (<a href='/en-us/products/primary-antibodies/rhogdi-antibody-epr3772-ab108977'>ab108977</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ARHGDIA knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human ARHGDIA (RhoGDI) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-arhgdia-rhogdi-knockout-hek-293t-cell-line-ab266447'>ab266447</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 23 kDa,27 kDa,30 kDa,62 kDa

Observed band size: 27 kDa,30 kDa,70 kDa

false

Western blot - Human ARHGDIA (RhoGDI) knockout HEK-293T cell lysate (AB257356)
  • WB

Lab

Western blot - Human ARHGDIA (RhoGDI) knockout HEK-293T cell lysate (AB257356)

Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : ARHGDIA knockout HEK293T cell lysate (20 ug)
Lane 3 : Jurkat cell lysate (20 ug)
Lane 4 : HeLa cell lysate (20 ug)

ab133248 was shown to specifically react with RhoGDI in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266447 (knockout cell lysate ab257356) was used. Wild-type and RhoGDI knockout samples were subjected to SDS-PAGE. ab133248 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-RhoGDI antibody [EPR3773] (<a href='/en-us/products/primary-antibodies/rhogdi-antibody-epr3773-ab133248'>ab133248</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ARHGDIA knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human ARHGDIA (RhoGDI) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-arhgdia-rhogdi-knockout-hek-293t-cell-line-ab266447'>ab266447</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

HeLa cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 23 kDa

Observed band size: 27 kDa

false

Sanger Sequencing - Human ARHGDIA (RhoGDI) knockout HEK-293T cell lysate (AB257356)
  • Sanger seq

Unknown

Sanger Sequencing - Human ARHGDIA (RhoGDI) knockout HEK-293T cell lysate (AB257356)

Allele-2 : 1 bp deletion in exon2

Sanger Sequencing - Human ARHGDIA (RhoGDI) knockout HEK-293T cell lysate (AB257356)
  • Sanger seq

Unknown

Sanger Sequencing - Human ARHGDIA (RhoGDI) knockout HEK-293T cell lysate (AB257356)

Allele-1 : Insertion of the selection cassette in exon2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and Insertion of the selection cassette in exon2.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257356-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human ARHGDIA knockout HEK293T cell lysate", "number":"AB257356-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257356-CMP02", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
ARHGDIA
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

RhoGDI also known as Rho GDP-dissociation inhibitor 1 is a regulatory protein with a mass of approximately 23 kDa. It primarily inhibits the dissociation of GDP from Rho GTPases such as RhoA Rac1 and Cdc42. This protein plays an important role in controlling the cycling between active GTP-bound and inactive GDP-bound states of small GTPases. RhoGDI shows expression in numerous tissues highlighting its widespread regulatory functions in cell signaling.
Biological function summary

RhoGDI modulates the activity of Rho family GTPases—a group responsible for organizing the actin cytoskeleton which determines cell shape polarity and movement. It does not operate alone; rather it is part of a complex with membrane-bound and cytosolic proteins offering a shuttle service to balance GTPases between cellular locations. By doing so RhoGDI influences processes like cell migration and the cell cycle integral to maintaining proper cellular architecture.

Pathways

Scientists recognize RhoGDI’s participation in key signaling pathways particularly the Rho GTPase cycle and actin cytoskeleton organization. It interacts with proteins like ROCK and PAK within these pathways to achieve precise control of cytoskeletal dynamics. RhoGDI coordinates with these proteins to play a central role in cellular responses to external stimuli impacting processes like wound healing and cellular development.

RhoGDI has associations with cancer and neurodegenerative diseases. Its deregulation can disrupt the Rho GTPase pathways leading to uncontrolled cell proliferation in cancers such as breast and prostate cancer. Additionally altered RhoGDI function affects neuronal growth and survival linking it to neurodegenerative conditions like Alzheimer’s disease. Within these contexts RhoGDI interacts with other proteins such as Rac1 and Cdc42 to influence disease progression and pathology.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB133248

Anti-RhoGDI antibody [EPR3773]

RabMAb|Recombinant|KO Validated

Western blot - Anti-RhoGDI antibody [EPR3773] (AB133248)

  • 5
  • 1
Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

  • 0
  • 0
Proteins & Peptides

AB288799

Recombinant Human VCAM1 protein (Active)

Premium Bioactive

Biological Activity - Recombinant Human VCAM1 protein (Active) (AB288799)

  • 0
  • 0
Proteins & Peptides

AB180278

Recombinant Human EXOSC8 protein

SDS-PAGE - Recombinant Human EXOSC8 protein (AB180278)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com