Human Arhgef1 (p115-RhoGEF) knockout HEK-293T cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
ARHGEF1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp deletion in exon3.
View Alternative Names
115 kD protein, 115 kDa guanine nucleotide exchange factor, ARHG1_HUMAN, ARHGEF 1, GEF 1, LBCL 2, LSC, Lsc homolog, Rho guanine nucleotide exchange factor (GEF) 1, Rho guanine nucleotide exchange factor 1, Sub1.5, p115-RhoGEF, p115RhoGEF
- WB
Lab
Western blot - Human Arhgef1 (p115-RhoGEF) knockout HEK-293T cell lysate (AB257842)
Lane 1 : Wild-type HEK-293T Scraped cell lysate 20 μg
Lane 2 : ARHGEF1 knockout HEK-293T Scraped cell lysate 20 μg
Lane 3 : HeLa cell lysate 20 μg
Lane 4 : U-2 OS cell lysate 20 μg
False colour image of Western blot : Anti-p115-RhoGEF antibody staining at 0.04 μg/ml, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab223759 was shown to bind specifically to p115-RhoGEF. A band was observed at 85 kDa in wild-type y cell lysates with no signal observed at this size in Arhgef1 knockout cell line ab266667 (knockout cell lysate ab257842). To generate this image, wild-type and Arhgef1 knockout y cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-p115-RhoGEF antibody (<a href='/en-us/products/primary-antibodies/p115-rhogef-antibody-ab223759'>ab223759</a>) at 0.04 µg/mL
Lane 1:
Wild-type HEK-293T Scraped cell lysate at 20 µg
Lane 2:
ARHGEF1 knockout HEK-293T Scraped cell lysate at 20 µg
Lane 2:
Western blot - Human Arhgef1 (p115-RhoGEF) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-arhgef1-p115-rhogef-knockout-hek-293t-cell-line-ab266667'>ab266667</a>)
Lane 3:
HeLa cell lysate at 20 µg
Lane 4:
U-2 OS cell lysate at 20 µg
Predicted band size: 102 kDa
Observed band size: 85 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human Arhgef1 (p115-RhoGEF) knockout HEK-293T cell lysate (AB257842)
Homozygous : 2 bp deletion in exon3
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
P115-RhoGEF plays an important role in regulating actin cytoskeleton dynamics and transmitting signals from G-protein-coupled receptors. The protein is part of a signaling complex that includes heterotrimeric G proteins. Through its influence on the actin cytoskeleton p115-RhoGEF helps control changes in cell shape and movement. These activities contribute significantly to various cellular processes including differentiation and migration.
Pathways
P115-RhoGEF is an essential component in the RhoA signaling pathway. This pathway regulates cytoskeletal dynamics and has critical implications for processes like cell adhesion and migration. p115-RhoGEF collaborates with other proteins such as Gα12/13 subunits to mediate its signaling effects. Besides the RhoA pathway p115-RhoGEF also interacts with the PI3K/Akt pathway influencing cellular survival and apoptosis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB266667
Human Arhgef1 (p115-RhoGEF) knockout HEK-293T cell line
Advanced Validation
- 0
- 0
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com