Human ARL13B knockout HeLa cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
- Sanger seq
Unknown
Sanger Sequencing - Human ARL13B knockout HeLa cell lysate (AB257260)
Allele-2 : Insertion of the selection cassette in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human ARL13B knockout HeLa cell lysate (AB257260)
Allele-1 : 5 bp deletion in exon1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ARL13B plays an essential role in the formation and maintenance of primary cilia which are key to cell signaling and sensory processing. ARL13B functions as part of a multiprotein complex within the ciliary membrane essential for the localization and distribution of other ciliary proteins. Through its GTPase activity ARL13B regulates ciliary dynamics and signaling pathways that are fundamental to proper cellular function and development.
Pathways
ARL13B modulates the Sonic Hedgehog (Shh) and Wnt signaling pathways both critical in developmental processes and tissue homeostasis. ARL13B interacts with several proteins in these pathways to control signal transduction such as Gli proteins in the Shh pathway and various cytoplasmic and nuclear factors in the Wnt pathway. ARL13B's role as a signaling regulator highlights its importance in maintaining cellular communication and function.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com