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AB263096

Human ARL8B knockout HeLa cell lysate

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ARL8B KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

View Alternative Names

ADP ribosylation factor like 10C, ADP ribosylation factor like 8B, ADP-ribosylation factor-like protein 10C, ADP-ribosylation factor-like protein 8B, ARL10C, ARL8B_HUMAN, FLJ10702, Gie1, Novel small G protein indispensable for equal chromosome segregation 1

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Sanger Sequencing - Human ARL8B knockout HeLa cell lysate (AB263096)
  • Sanger seq

Unknown

Sanger Sequencing - Human ARL8B knockout HeLa cell lysate (AB263096)

Homozygous : 1 bp insertion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

Disease

Adenocarcinoma

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ARL8B
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ARL8B also known as ADP-ribosylation factor-like protein 8B is a small GTPase with an approximate molecular mass of 22 kDa. It mainly localizes to the lysosomal membrane. This protein plays an important role in the regulation of intracellular trafficking and lysosome positioning. ARL8B is expressed across many tissues suggesting its involvement in broad cellular functions.
Biological function summary

ARL8B helps manage the proper function of lysosomes by facilitating their movement and fusion with other cellular compartments. It forms a complex with other proteins such as PLEKHM2 and HOPS which are necessary for tethering events during lysosomal trafficking. This protein contributes to cellular processes such as autophagy and antigen presentation influencing the cell's ability to maintain homeostasis and effectively respond to external stimuli.

Pathways

ARL8B participates in the autophagy pathway and plays a part in the lysosome-related processes. It works closely with proteins like the kinesin motor proteins which drive the movement of lysosomes along microtubules. Through these interactions ARL8B coordinates with other autophagy-related proteins to ensure efficient degradation of cellular debris and recycling of components which are important for cellular health and function.

ARL8B has links to neurodegenerative diseases and immune system dysfunctions. Dysregulation of lysosomal positioning and movement caused by abnormal ARL8B function can lead to conditions such as Charcot-Marie-Tooth disease and impaired immune responses. Furthermore ARL8B interacts with proteins like Rab7a which can have implications in disease pathways emphasizing its significant role in maintaining cellular balance.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

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