Human ARNTL (BMAL1) knockout HeLa cell lysate
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(1 Publication)
BMAL1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon10.
View Alternative Names
ARNT like protein 1 brain and muscle, Arntl, Aryl hydrocarbon receptor nuclear translocator like, Aryl hydrocarbon receptor nuclear translocator-like protein 1, BMAL1_HUMAN, BMAL1c, Basic helix loop helix PAS orphan MOP3, Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1, CG8727 PA, Class E basic helix-loop-helix protein 5, JAP 3, MGC47515, MOP 3, Member of PAS protein 3, Member of PAS superfamily 3, PAS domain-containing protein 3, PASD 3, TIC, bHLH-PAS protein JAP3, bHLHe5, cycle
- WB
Lab
Western blot - Human ARNTL (BMAL1) knockout HeLa cell lysate (AB258314)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : ARNTL knockout HeLa cell lysate 20 μg
Lane 3 : SH-SY5Y cell lysate 20 μg
Lane 4 : Huh7 cell lysate 20 μg
Lanes 1 - 4 : Merged signal (red and green). Green - Anti-β1-Adrenergic Receptor antibody observed at 76 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
Anti-β1-Adrenergic Receptor antibody was shown to react with β1-Adrenergic Receptor in wild-type HeLa cells in Western blot with loss of signal observed in ARNTL knockout cell line ab264701 (ARNTL knockout cell lysate ab258314). Wild-type HeLa and ARNTL knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with Anti-β1-Adrenergic Receptor antibody and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.
Lane 1:
Anti-ß1-Adrenergic Receptor antibody at 1/1000 dilution
Lanes 2 - 4:
Anti-β1-Adrenergic Receptor antibody at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ARNTL knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ARNTL (BMAL1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-arntl-bmal1-knockout-hela-cell-line-ab264701'>ab264701</a>)
Lane 3:
SH-SY5Y cell lysate at 20 µg
Lane 4:
Huh7 cell lysate at 20 µg
false
- Sanger seq
Unknown
Sanger Sequencing - Human ARNTL (BMAL1) knockout HeLa cell lysate (AB258314)
Homozygous : 1 bp deletion in exon10
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Publications (1)
Recent publications for all applications. Explore the full list and refine your search
Polish journal of pathology : official journal of the Polish Society of Pathologists 73:99-110 PubMed35979756
2022
Applications
Unspecified application
Species
Unspecified reactive species
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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