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AB257282

Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate

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ARRB2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4.
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Western blot - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate (AB257282)
  • WB

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Western blot - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate (AB257282)

Lane 1 : Wild-type HEK293T cell lysate (20 μg)

Lane 2 : ARRB2 knockout HEK293T cell lysate (20 μg)

Lane 3 : Mouse brain tissue lysate (20 μg)

Lane 4 : Human lung tissue lysate (20 μg)

Lanes 1-4 : Merged signal (red and green). Green - ab54790 observed at 48-55 kDa. Red - loading control, ab181602 observed at 37 kDa.

ab54790 Anti-Beta Arrestin 2 antibody was shown to specifically react with Beta Arrestin 2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266116 (knockout cell lysate ab257282) was used. Wild-type and Beta Arrestin 2 knockout samples were subjected to SDS-PAGE. ab54790 and Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Beta Arrestin 2 antibody [3G1] (<a href='/en-us/products/primary-antibodies/beta-arrestin-2-antibody-3g1-ab54790'>ab54790</a>) at 1/500 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ARRB2 knockout HEK293T cell lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Human lung tissue lysate at 20 µg

Predicted band size: 46 kDa

Observed band size: 48-55 kDa

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Sanger Sequencing - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate (AB257282)
  • Sanger seq

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Sanger Sequencing - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate (AB257282)

Homozygous : 1 bp deletion in exon4

Western blot - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate (AB257282)
  • WB

Supplier Data

Western blot - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate (AB257282)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, ab314213 was shown to bind specifically to Beta Arrestin 2. A band was observed at 50 kDa and 37kDa in wild-type 293T cell lysates whereas loss of signal was observed in the ARRB2 knockout cell line ab266116 (knockout cell lysate ab257282).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Beta Arrestin 2 antibody [EPR25410-70] (<a href='/en-us/products/primary-antibodies/beta-arrestin-2-antibody-epr25410-70-ab314213'>ab314213</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell lysate (ab257282) at 20 µg

Lane 3:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HepG2 (human hepatocellar carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 37 kDa,50 kDa

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Exposure time: 180s

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon4.

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Complementary Products

Cell Lines & Lysates

AB266116

Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell line

Advanced Validation

Western blot - Human ARRB2 (Beta Arrestin 2) knockout HEK-293T cell line (AB266116)

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Primary Antibodies

AB206972

Anti-Beta Arrestin 2 + Beta Arrestin 1 antibody [EPR22073]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-Beta Arrestin 2 + Beta Arrestin 1 antibody [EPR22073] (AB206972)

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Proteins & Peptides

AB259412

Recombinant human VEGF 165A protein (Active)

Premium Bioactive

Mass Spectrometry - Recombinant human VEGF 165A protein (Active) (AB259412)

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Proteins & Peptides

AB132309

Recombinant Human Beta Arrestin 2 protein

SDS-PAGE - Recombinant Human Beta Arrestin 2 protein (AB132309)

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  • 0
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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ARRB2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-arrestin 2 also known as arrb2 or b-arrestin mechanically functions to regulate G protein-coupled receptor (GPCR) signaling. This protein has a molecular mass of approximately 47 kDa and is ubiquitously expressed across various tissues including the brain lungs and heart. Beta-arrestin 2 works by binding to phosphorylated GPCRs facilitating receptor desensitization and endocytosis which stops the receptor's signaling activity and mediates receptor internalization.
Biological function summary

Beta-arrestin 2 plays an important role in mediating signal transduction pathways beyond GPCR desensitization including those involved in cell communication and cellular response regulation. Beta-arrestin 2 interacts with multiple signaling proteins to form complexes that facilitate signaling pathways notably influencing MAPK (Mitogen-Activated Protein Kinase) pathways. It provides a scaffold for downstream signaling molecules affecting cell signaling outcomes.

Pathways

Beta-arrestin 2 significantly participates in two important biological pathways: the MAPK pathway and the Wnt signaling pathway. Through these pathways beta-arrestin 2 interacts with signaling proteins such as ERK1/2 (Extracellular signal-Regulated Kinases) and dishevelled proteins. These interactions highlight beta-arrestin 2's ability to modulate signal strength and duration contributing to diverse cellular responses.

Beta-arrestin 2 is implicated in conditions like heart failure and certain cancers. The aberrant regulation of beta-arrestin 2-related pathways can lead to uncontrolled cell proliferation or inadequate heart responses. Additionally beta-arrestin 2 is connected to proteins like GPCR kinases in the context of heart failure and MAPK proteins within oncogenic processes underlining its potential as a therapeutic target.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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