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AB257283

Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell lysate

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ARRB2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon4.
3 Images
Western blot - Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell lysate (AB257283)
  • WB

Lab

Western blot - Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell lysate (AB257283)

Lane 1 : Wild-type HepG2 cell lysate (20 ug)
Lane 2 : ARRB2 knockout HepG2 cell lysate (20 ug)
Lane 3 : HeLa cell lysate (20 ug)
Lane 4 : Human Lung tissue lysate (20 ug)

Lanes 1 - 4 : Merged signal (red and green). Green - ab54790 observed at 50 kDa. Red - loading control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

ab54790 was shown to react with Beta Arrestin 2 in wild-type HepG2 cells in Western blot with loss of signal observed in ARRB2 knockout cell line ab262320 (knockout cell lysate ab257283). Wild-type HepG2 and ARRB2 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab54790 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at 1 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-Beta Arrestin 2 antibody [3G1] (<a href='/en-us/products/primary-antibodies/beta-arrestin-2-antibody-3g1-ab54790'>ab54790</a>) at 1 µg/mL

Lane 1:

Wild-type HepG2 cell lysate at 20 µg

Lane 2:

ARRB2 knockout HepG2 cell lysate at 20 µg

Lane 2:

Western blot - Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell line (<a href='/en-us/products/cell-lines/human-arrb2-beta-arrestin-2-knockout-hep-g2-cell-line-ab262320'>ab262320</a>)

Lane 3:

HeLa cell lysate at 20 µg

Lane 4:

Human Lung tissue lysate at 20 µg

Predicted band size: 46 kDa

Observed band size: 50 kDa

false

Western blot - Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell lysate (AB257283)
  • WB

Lab

Western blot - Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell lysate (AB257283)

Lane 1 : Wild-type HepG2 cell lysate (20µg)

Lane 2 : ARRB2 knockout HepG2 cell lysate (20µg)

Lane 3 : A549 cell lysate (20µg)

Lane 4 : K562 cell lysate (20µg)

Lanes 1- 4 : Merged signal (red and green). Green - ab54790 observed at 55 kDa. Red - loading control ab181602 observed at 37 kDa.

ab54790 Anti-Beta Arrestin 2 antibody was shown to specifically react with ARRB2 in wild-type HepG2 cells in western blot. Loss of signal was observed when knockout cell line ab262320 (knockout cell lysate ab257283) was used. Wild-type and ARRB2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab54790 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Beta Arrestin 2 antibody [3G1] (<a href='/en-us/products/primary-antibodies/beta-arrestin-2-antibody-3g1-ab54790'>ab54790</a>) at 1/500 dilution

Lane 1:

Wild-type HepG2 cell lysate at 20 µg

Lane 2:

ARRB2 knockout HepG2 cell lysate at 20 µg

Lane 2:

Western blot - Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell line (<a href='/en-us/products/cell-lines/human-arrb2-beta-arrestin-2-knockout-hep-g2-cell-line-ab262320'>ab262320</a>)

Lane 3:

A549 cell lysate at 20 µg

Lane 4:

K562 cell lysate at 20 µg

Predicted band size: 46 kDa

Observed band size: 55 kDa

false

Sanger Sequencing - Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell lysate (AB257283)
  • Sanger seq

Unknown

Sanger Sequencing - Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell lysate (AB257283)

Homozygous : 1 bp deletion in exon4

Key facts

Cell type

Hep G2

Species or organism

Human

Tissue

Liver

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon4.

Disease

Hepatocellular Carcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ARRB2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-arrestin 2 also known as arrb2 or b-arrestin mechanically functions to regulate G protein-coupled receptor (GPCR) signaling. This protein has a molecular mass of approximately 47 kDa and is ubiquitously expressed across various tissues including the brain lungs and heart. Beta-arrestin 2 works by binding to phosphorylated GPCRs facilitating receptor desensitization and endocytosis which stops the receptor's signaling activity and mediates receptor internalization.
Biological function summary

Beta-arrestin 2 plays an important role in mediating signal transduction pathways beyond GPCR desensitization including those involved in cell communication and cellular response regulation. Beta-arrestin 2 interacts with multiple signaling proteins to form complexes that facilitate signaling pathways notably influencing MAPK (Mitogen-Activated Protein Kinase) pathways. It provides a scaffold for downstream signaling molecules affecting cell signaling outcomes.

Pathways

Beta-arrestin 2 significantly participates in two important biological pathways: the MAPK pathway and the Wnt signaling pathway. Through these pathways beta-arrestin 2 interacts with signaling proteins such as ERK1/2 (Extracellular signal-Regulated Kinases) and dishevelled proteins. These interactions highlight beta-arrestin 2's ability to modulate signal strength and duration contributing to diverse cellular responses.

Beta-arrestin 2 is implicated in conditions like heart failure and certain cancers. The aberrant regulation of beta-arrestin 2-related pathways can lead to uncontrolled cell proliferation or inadequate heart responses. Additionally beta-arrestin 2 is connected to proteins like GPCR kinases in the context of heart failure and MAPK proteins within oncogenic processes underlining its potential as a therapeutic target.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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