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ARRB2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon4.

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Images

Western blot - Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell lysate (AB257283), expandable thumbnail
  • Western blot - Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell lysate (AB257283), expandable thumbnail
  • Sanger Sequencing - Human ARRB2 (Beta Arrestin 2) knockout Hep G2 cell lysate (AB257283), expandable thumbnail

Key facts

Cell type
Hep G2
Species or organism
Human
Tissue
Liver
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon4.

Alternative names

ARB 2, ARR 2, ARRB2_HUMAN, Arrestin 3, Arrestin beta-2, BARR2, Beta-arrestin-2, DKFZp686L0365, HGNC:712

What's included?

1 Kit
Components
Human ARRB2 knockout HepG2 cell lysate
1 x 100 µg
Human wild-type HepG2 cell lysate
1 x 100 µg

Recommended products

ARRB2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon4.

Key facts

Cell type
Hep G2
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon4.
Disease
Hepatocellular Carcinoma
Concentration
Loading...

Properties

Gene name
ARRB2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

Beta-arrestin 2 also known as arrb2 or b-arrestin mechanically functions to regulate G protein-coupled receptor (GPCR) signaling. This protein has a molecular mass of approximately 47 kDa and is ubiquitously expressed across various tissues including the brain lungs and heart. Beta-arrestin 2 works by binding to phosphorylated GPCRs facilitating receptor desensitization and endocytosis which stops the receptor's signaling activity and mediates receptor internalization.

Biological function summary

Beta-arrestin 2 plays an important role in mediating signal transduction pathways beyond GPCR desensitization including those involved in cell communication and cellular response regulation. Beta-arrestin 2 interacts with multiple signaling proteins to form complexes that facilitate signaling pathways notably influencing MAPK (Mitogen-Activated Protein Kinase) pathways. It provides a scaffold for downstream signaling molecules affecting cell signaling outcomes.

Pathways

Beta-arrestin 2 significantly participates in two important biological pathways: the MAPK pathway and the Wnt signaling pathway. Through these pathways beta-arrestin 2 interacts with signaling proteins such as ERK1/2 (Extracellular signal-Regulated Kinases) and dishevelled proteins. These interactions highlight beta-arrestin 2's ability to modulate signal strength and duration contributing to diverse cellular responses.

Associated diseases and disorders

Beta-arrestin 2 is implicated in conditions like heart failure and certain cancers. The aberrant regulation of beta-arrestin 2-related pathways can lead to uncontrolled cell proliferation or inadequate heart responses. Additionally beta-arrestin 2 is connected to proteins like GPCR kinases in the context of heart failure and MAPK proteins within oncogenic processes underlining its potential as a therapeutic target.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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