Human ASH1L knockout HeLa cell lysate
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ASH1L KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 3 and 1 bp insertion in exon 3.
View Alternative Names
ASH 1, ASH1-like protein, ASH1L1, ASH1L_HUMAN, Absent Small or Homeotic Like, Absent small and homeotic disks protein 1 homolog, Ash1 (absent small or homeotic) like, FLJ10504, KIAA1420, KMT2H / ASH1, Lysine N-methyltransferase 2H, Probable histone-lysine N-methyltransferase ASH1L, ash1 (absent small or homeotic 1), huASH1
- Sanger seq
Unknown
Sanger Sequencing - Human ASH1L knockout HeLa cell lysate (AB263097)
Allele-1 : 1 bp deletion in exon 3
- Sanger seq
Unknown
Sanger Sequencing - Human ASH1L knockout HeLa cell lysate (AB263097)
Allele-2 : 1 bp insertion in exon 3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ASH1L contributes to various processes involving transcriptional regulation and chromatin modification. This protein functions as part of the Set1/Ash1 complex where it influences gene expression by specifically methylating histone H3 at lysine 36 (H3K36). The methylation of this residue is associated with active transcription and is important for the proper regulation of genes involved in development and differentiation. ASH1L-mediated modifications thereby facilitate the global maintenance of the active chromatin state essential for cellular processes.
Pathways
ASH1L is engaged in pathways related to histone modification and transcriptional regulation. It functions alongside proteins in the Wnt signaling pathway which plays a vital role in regulating tissue-specific transcription programs. ASH1L can alternatively interact with other histone methyltransferases like the NSD family which share similar functions in chromatin dynamics and are involved in transcription elongation and chromatin remodeling.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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