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AB257359

Human ATAD2 knockout HeLa cell lysate

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ATAD2 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1.

View Alternative Names

ATAD2, PRO2000, ANCCA, ATPase family AAA domain-containing protein 2, L16, AAA nuclear coregulator cancer-associated protein

2 Images
Western blot - Human ATAD2 knockout HeLa cell lysate (AB257359)
  • WB

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Western blot - Human ATAD2 knockout HeLa cell lysate (AB257359)

Lane 1 : Wild-type HeLa cell lysate (20µg)

Lane 2 : ATAD2 knockout HeLa cell lysate (20µg)

Lanes 1- 2 : Merged signal (red and green). Green - ab176319 observed at 200 kDa. Red - loading control, ab7291 observed at 50 kDa.

ab176319 Recombinant Anti-Calnexin antibody [EPR3633(2)] was shown to specifically react with ATAD2 in wild-type HeLa cells in western blot. The band observed in the knockout cell line ab264957 (knockout cell lysate ab257359) lane below 200kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and ATAD2 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab176319 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATAD2 antibody [EPR12730] (<a href='/en-us/products/primary-antibodies/atad2-antibody-epr12730-ab176319'>ab176319</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ATAD2 knockout HeLa cell lysate at 20 µg

Predicted band size: 159 kDa

Observed band size: 200 kDa

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Sanger Sequencing - Human ATAD2 knockout HeLa cell lysate (AB257359)
  • Sanger seq

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Sanger Sequencing - Human ATAD2 knockout HeLa cell lysate (AB257359)

Homozygous : 7 bp deletion in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 7 bp deletion in exon 1.

Disease

Adenocarcinoma

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Complementary Products

Primary Antibodies

AB242068

violetFluor™ 450 Anti-CD127 antibody [A7R34]

Flow Cytometry - violetFluor™ 450 Anti-CD127 antibody [A7R34] (AB242068)

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Primary Antibodies

AB93527

FITC Anti-SLAM / CD150 antibody [9D1]

Flow Cytometry - FITC Anti-SLAM / CD150 antibody [9D1] (AB93527)

  • 0
  • 0
Primary Antibodies

AB302975

Alexa Fluor® 488 Anti-c-Kit antibody [EPR25707-134]

RabMAb|Recombinant

  • 0
  • 0
Primary Antibodies

AB320068

Alexa Fluor® 488 Anti-CD36 antibody [EPR22509-40]

BOND RX™ Validated|Recombinant|RabMAb|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 488 Anti-CD36 antibody [EPR22509-40] (AB320068)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.</p>" } } }
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Product details

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257359-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB257359-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human ATAD2 knockout HeLa cell lysate", "number":"AB257359-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
ATAD2
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATAD2 also known as ATPase family AAA domain-containing protein 2 is a chromatin-associated AAA ATPase. This protein weighs approximately 157 kDa and functions mainly in the cell nucleus. ATAD2 is widely expressed in various tissues with high levels found in the testis indicating its possible role in testicular functions. It shows ATPase activity involving the hydrolysis of ATP which provides energy for chromatin remodeling processes.
Biological function summary

ATAD2 plays a critical role in transcription regulation by remodeling chromatin structure. As part of a complex it interacts with histone acetylation marks on chromatin therefore influencing gene expression. The protein is often involved in the regulation of genes relevant to cell cycle progression. Its interaction with transcriptional regulators highlights its importance in controlling proliferation and other cellular processes.

Pathways

The role of ATAD2 becomes essential in pathways like the cell cycle and the DNA damage response. Through these pathways it interacts with key proteins such as MYC a known regulator of cell growth and it influences the expression of genes involved in cell cycle control. It is important in maintaining genomic stability by ensuring proper chromatin dynamics during DNA replication and repair.

Aberrant expression of ATAD2 associates with cancer progression particularly in breast cancer where it is highly expressed in MCF7 cell lines. It is also implicated in hepatocellular carcinoma where it interacts with cancer-related proteins such as p53 which is a well-known tumor suppressor. These interactions suggest that ATAD2 serves as a potential target for cancer therapies focused on disrupting its interactions or functions in tumor biology.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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