ATF3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp deletion in exon2 and 4 bp deletion in exon2.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp deletion in exon2 and 4 bp deletion in exon2.
Alternative names
ATF3_HUMAN, ATF3deltaZip2, ATF3deltaZip2c, ATF3deltaZip3, Activating transcription factor 3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3
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ATF3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp deletion in exon2 and 4 bp deletion in exon2.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp deletion in exon2 and 4 bp deletion in exon2.
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- ATF3
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATF3 also known as Activating Transcription Factor 3 functions primarily as a stress-responsive transcription factor. This protein has a molecular weight of about 21 kDa and is part of the ATF/CREB family of transcription factors. ATF3 is expressed in various tissues and is known for its role in stress response and regulation of gene expression. The expression levels change in response to cellular stress and it may interact with several other transcription factors to modulate gene expression profiles.
- Biological function summary
ATF3 acts as a regulator of cellular stress responses and can influence cell cycle arrest and apoptosis. It does not form a large complex but interacts with diverse partners to execute its functions. Studies often use HCT116 cells to investigate ATF3's role where it can serve as a marker of cellular stress and inflammation. The protein regulates genes involved in maintaining homeostasis in stressed cells.
- Pathways
ATF3 is involved in stress response and apoptotic pathways. In the context of apoptosis ATF3 interacts with proteins like p53 and Jun which are important for inducing cell cycle arrest and programmed cell death. In stress response pathways ATF3 modifies transcriptional activity in reaction to various stress signals providing a checkpoint for damaged cells before repairing or entering programmed death.
- Associated diseases and disorders
ATF3 relates to cancer and cardiovascular diseases. In cancer abnormal expression of ATF3 can influence tumor growth and metastasis often acting through its interactions with proteins like NF-kB. Additionally in cardiovascular disorders ATF3's role in regulating stress responses underpins its involvement in conditions linked to inflammation and atherosclerosis again with NF-kB signaling being a relevant pathway of interaction. Researchers continue to study ATF3 to uncover therapeutic targets for these and other diseases.
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5 product images
Western blot - Human ATF3 knockout A549 cell lysate (ab257075)
Lane 1: Wild-type A549 cell lysate (20 μg)Lane 2: ATF3 knockout A549 cell lysate (20 μg)
Lanes 1-2: Merged signal (red and green). Green - Anti-ATF3 antibody [EPR19488] - ChIP Grade ab207434 observed at 21 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-ATF3 antibody [EPR19488] - ChIP Grade ab207434 Anti-ATF3 antibody [EPR19488] - ChIP Grade was shown to specifically react with ATF3 in wild-type A549 cells. Loss of signal was observed when knockout cell line Human ATF3 knockout A549 cell line ab266955 (knockout cell lysate ab257075) was used. Wild-type and ATF3 knockout samples were subjected to SDS-PAGE. Anti-ATF3 antibody [EPR19488] - ChIP Grade ab207434 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ATF3 antibody [EPR19488] - ChIP Grade (Anti-ATF3 antibody [EPR19488] - ChIP Grade ab207434) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: ATF3 knockout A549 cell lysate at 20 µg
Lane 2: Western blot - Human ATF3 knockout A549 cell line (Human ATF3 knockout A549 cell line ab266955)
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Western blot - Human ATF3 knockout A549 cell lysate (ab257075)
Lane 1: Wild-type A549 cell lysate (20 μg)Lane 2: ATF3 knockout A549 cell lysate (20 μg)
Lanes 1-2: Merged signal (red and green). Green - Anti-ATF3 antibody [EPR22610-19] - ChIP Grade ab254268 observed at 21 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 37 kDa.
Anti-ATF3 antibody [EPR22610-19] - ChIP Grade ab254268 Recombinant Anti-ATF3 antibody [EPR22610-19] was shown to specifically react with ATF3 in wild-type A549 cells. Loss of signal was observed when knockout cell line Human ATF3 knockout A549 cell line ab266955 (knockout cell lysate ab257075) was used. Wild-type and ATF3 knockout samples were subjected to SDS-PAGE. Anti-ATF3 antibody [EPR22610-19] - ChIP Grade ab254268 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes: Western blot - Anti-ATF3 antibody [EPR22610-19] - ChIP Grade (Anti-ATF3 antibody [EPR22610-19] - ChIP Grade ab254268) at 1/1000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: ATF3 knockout A549 cell lysate at 20 µg
Lane 2: Western blot - Human ATF3 knockout A549 cell line (Human ATF3 knockout A549 cell line ab266955)
Performed under reducing conditions.
Predicted band size: 21 kDa
Observed band size: 21 kDa
Sanger Sequencing - Human ATF3 knockout A549 cell lysate (ab257075)
Allele-1: 14 bp deletion in exon2
Sanger Sequencing - Human ATF3 knockout A549 cell lysate (ab257075)
Allele-2: 4 bp deletion in exon2
Sanger Sequencing - Human ATF3 knockout A549 cell lysate (ab257075)
Allele-3: 1 bp deletion in exon2
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