Human ATF3 knockout A549 cell lysate
Be the first to review this product! Submit a review
|
(0 Publication)
ATF3 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 14 bp deletion in exon2 and 1 bp deletion in exon2 and 4 bp deletion in exon2.
View Alternative Names
ATF3_HUMAN, ATF3deltaZip2, ATF3deltaZip2c, ATF3deltaZip3, Activating transcription factor 3, Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3
- WB
Lab
Western blot - Human ATF3 knockout A549 cell lysate (AB257075)
Lane 1 : Wild-type A549 cell lysate (20 μg)
Lane 2 : ATF3 knockout A549 cell lysate (20 μg)
Lanes 1-2 : Merged signal (red and green). Green - ab207434 observed at 21 kDa. Red - loading control ab8245 observed at 37 kDa.
ab207434 Anti-ATF3 antibody [EPR19488] - ChIP Grade was shown to specifically react with ATF3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266955 (knockout cell lysate ab257075) was used. Wild-type and ATF3 knockout samples were subjected to SDS-PAGE. ab207434 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ATF3 antibody [EPR19488] - ChIP Grade (<a href='/en-us/products/primary-antibodies/atf3-antibody-epr19488-chip-grade-ab207434'>ab207434</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
ATF3 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human ATF3 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-atf3-knockout-a549-cell-line-ab266955'>ab266955</a>)
Predicted band size: 21 kDa
Observed band size: 21 kDa
false
- WB
Lab
Western blot - Human ATF3 knockout A549 cell lysate (AB257075)
Lane 1 : Wild-type A549 cell lysate (20 μg)
Lane 2 : ATF3 knockout A549 cell lysate (20 μg)
Lanes 1-2 : Merged signal (red and green). Green - ab254268 observed at 21 kDa. Red - loading control ab8245 observed at 37 kDa.
ab254268 Recombinant Anti-ATF3 antibody [EPR22610-19] was shown to specifically react with ATF3 in wild-type A549 cells. Loss of signal was observed when knockout cell line ab266955 (knockout cell lysate ab257075) was used. Wild-type and ATF3 knockout samples were subjected to SDS-PAGE. ab254268 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ATF3 antibody [EPR22610-19] - ChIP Grade (<a href='/en-us/products/primary-antibodies/atf3-antibody-epr22610-19-chip-grade-ab254268'>ab254268</a>) at 1/1000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
ATF3 knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human ATF3 knockout A549 cell line (<a href='/en-us/products/cell-lines/human-atf3-knockout-a549-cell-line-ab266955'>ab266955</a>)
Predicted band size: 21 kDa
Observed band size: 21 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ATF3 knockout A549 cell lysate (AB257075)
Allele-2 : 4 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human ATF3 knockout A549 cell lysate (AB257075)
Allele-1 : 14 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human ATF3 knockout A549 cell lysate (AB257075)
Allele-3 : 1 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATF3 acts as a regulator of cellular stress responses and can influence cell cycle arrest and apoptosis. It does not form a large complex but interacts with diverse partners to execute its functions. Studies often use HCT116 cells to investigate ATF3's role where it can serve as a marker of cellular stress and inflammation. The protein regulates genes involved in maintaining homeostasis in stressed cells.
Pathways
ATF3 is involved in stress response and apoptotic pathways. In the context of apoptosis ATF3 interacts with proteins like p53 and Jun which are important for inducing cell cycle arrest and programmed cell death. In stress response pathways ATF3 modifies transcriptional activity in reaction to various stress signals providing a checkpoint for damaged cells before repairing or entering programmed death.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB207434
Anti-ATF3 antibody [EPR19488] - ChIP Grade
RabMAb|Advanced Validation|Recombinant|KO Validated|20ul selling size
![Immunocytochemistry/ Immunofluorescence - Anti-ATF3 antibody [EPR19488] - ChIP Grade (AB207434)](https://content.abcam.com/products/images/atf3-antibody-epr19488-chip-grade-ab207434--immunocytochemistry-immunofluorescence-img87225.jpg)
- 5
- 1
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com