Human ATG16L1 knockout HeLa cell lysate
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ATG16L1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 22 bp deletion in exon1 and Insertion of the selection cassette in exon1.
View Alternative Names
A16L1_HUMAN, APG16-like 1, APG16L, APG16L beta, ATG16 autophagy related 16 like 1, ATG16 autophagy related 16-like 1 (S. cerevisiae), ATG16A, ATG16L, Atg16l1, Autophagy-related protein 16-1, FLJ00045, FLJ10035, FLJ10828, FLJ22677, IBD10, OTTHUMP00000164391, OTTHUMP00000164393, OTTHUMP00000165876, OTTHUMP00000165877, WD repeat domain 30, WDR30
- WB
Unknown
Western blot - Human ATG16L1 knockout HeLa cell lysate (AB256843)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : ATG16L1 knockout HeLa cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lane 4 : Daudi cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab233796 observed at 68 and 72 kDa. Red - loading control ab181602 observed at 37 kDa.
ab233796 Anti-ATG16L1 antibody [5H9A11] was shown to specifically react with ATG16L1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261772 (knockout cell lysate ab256843) was used. Wild-type and ATG16L1 knockout samples were subjected to SDS-PAGE. ab233796 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ATG16L1 antibody [5H9A11] (<a href='/en-us/products/primary-antibodies/atg16l1-antibody-5h9a11-ab233796'>ab233796</a>) at 1/500 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ATG16L1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ATG16L1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-atg16l1-knockout-hela-cell-line-ab261772'>ab261772</a>)
Lane 3:
Jurkat cell lysate at 20 µg
Lane 4:
Daudi cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa,72 kDa
false
- WB
Supplier Data
Western blot - Human ATG16L1 knockout HeLa cell lysate (AB256843)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : ATG16L1 knockout HeLa cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lane 4 : Daudi cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab187671 observed at 68 and 72 kDa. Red - loading control ab8245 observed at 37 kDa.
ab187671 Anti-ATG16L1 antibody [EPR15638] - N-terminal was shown to specifically react with ATG16L1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261772 (knockout cell lysate ab256843) was used. Wild-type and ATG16L1 knockout samples were subjected to SDS-PAGE. ab187671 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ATG16L1 antibody [EPR15638] - N-terminal (<a href='/en-us/products/primary-antibodies/atg16l1-antibody-epr15638-n-terminal-ab187671'>ab187671</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ATG16L1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ATG16L1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-atg16l1-knockout-hela-cell-line-ab261772'>ab261772</a>)
Lane 3:
Jurkat cell lysate at 20 µg
Lane 4:
Daudi cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 68 kDa
Observed band size: 68 kDa,72 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ATG16L1 knockout HeLa cell lysate (AB256843)
Allele-2 : 22 bp deletion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human ATG16L1 knockout HeLa cell lysate (AB256843)
Allele-1 : Insertion of the selection cassette in exon1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATG16L1 plays a role in mediating autophagy a vital cellular degradation process. It forms a complex with ATG5 and ATG12 necessary for the elongation of the autophagosome membrane. Apart from its role in autophagy ATG16L1 contributes to the regulation of innate immunity by influencing the secretion of inflammatory cytokines. Its presence is essential for maintaining cellular homeostasis and proper immune responses.
Pathways
ATG16L1 is important in pathways like autophagy and immunity. In the autophagy pathway it works alongside ATG5 and ATG12 to ensure the proper formation of autophagosomes which are structures that engulf and degrade unwanted cellular components. Furthermore in the immune response pathway it helps regulate inflammation by managing cytokine production and secretion showing interaction with proteins like NOD2.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB187671
Anti-ATG16L1 antibody [EPR15638] - N-terminal
RabMAb|Recombinant|KO Validated|Trial Size
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG16L1 antibody [EPR15638] - N-terminal (AB187671)](https://content.abcam.com/products/images/atg16l1-antibody-epr15638-n-terminal-ab187671--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img84248.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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