Human ATG16L2 knockout HeLa cell lysate
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ATG16L2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon6.
View Alternative Names
A16L2_HUMAN, APG16-like 2, ATG16 autophagy related 16-like 2, ATG16B, Autophagy-related protein 16-2, WD repeat-containing protein 80, WDR80, autophagy related 16-like 2 (S. cerevisiae)
- Sanger seq
Unknown
Sanger Sequencing - Human ATG16L2 knockout HeLa cell lysate (AB263098)
Homozygous : 1 bp deletion in exon6
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
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It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATG16L2 contributes to cellular homeostasis by supporting autophagic activity. It associates with the ATG5-ATG12 complex essential for the formation of autophagosomes. This interaction ensures the delivery of cytoplasmic content to lysosomes for degradation an important step in autophagic flux. The presence of ATG16L2 in immune cells suggests an additional role in modulating immune responses and maintaining balance during stress conditions.
Pathways
ATG16L2 is primarily involved in the autophagy and lysosomal degradation pathways. These pathways are important for maintaining cell survival under nutrient-deprived conditions. Within its pathways ATG16L2 interacts closely with proteins like LC3 which is necessary for autophagosome maturation and function. It's part of the larger network of proteins orchestrating the cellular response to metabolic stress and damage.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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