Human ATG7 knockout HeLa cell lysate
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ATG7 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, functional Homozygous: 41 dp deletion in exon 4.
View Alternative Names
1810013K23Rik, APG7 autophagy 7 like, APG7 autophagy 7-like (S. cerevisiae), APG7, S. cerevisiae, homolog of, APG7-like, APG7L, ATG12-activating enzyme E1 ATG7, ATG7 autophagy related 7 homolog, ATG7 autophagy related 7 homolog (S. cerevisiae), ATG7_HUMAN, Apg 7, Atg7l, Autophagy 7, S. cerevisiae, homolog of, Autophagy-related 7 (yeast), Autophagy-related protein 7, DKFZp434N0735, GSA 7, Ubiquitin-activating enzyme E1-like protein, Ubiquitin-like modifier-activating enzyme ATG7, hAGP7
- WB
Lab
Western blot - Human ATG7 knockout HeLa cell lysate (AB287353)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : ATG7 knockout HeLa cell lysate 20 μg
Lane 3 : HepG2 cell lysate 20 μg
Lane 4 : Jurkat cell lysate 20 μg
False colour image of Western blot : Anti-ATG7 antibody [EPR6251] staining at 1/10000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab133528 was shown to bind specifically to ATG7. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in ATG7 knockout cell line ab283307 (knockout cell lysate ab287353). To generate this image, wild-type and ATG7 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-ATG7 antibody [EPR6251] (<a href='/en-us/products/primary-antibodies/atg7-antibody-epr6251-ab133528'>ab133528</a>) at 1/10000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ATG7 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ATG7 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-atg7-knockout-hela-cell-line-ab283307'>ab283307</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
Jurkat cell lysate at 20 µg
Predicted band size: 77 kDa
Observed band size: 75 kDa
false
- WB
Lab
Western blot - Human ATG7 knockout HeLa cell lysate (AB287353)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : ATG7 knockout HeLa cell lysate 20 μg
Lane 3 : HepG2 cell lysate 20 μg
Lane 4 : Jurkat cell lysate 20 μg
False colour image of Western blot : Anti-ATG7 antibody [EP1759Y] staining at 1/100000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab52472 was shown to bind specifically to ATG7. A band was observed at 75 kDa in wild-type HeLa cell lysates with no signal observed at this size in ATG7 knockout cell line ab283307 (knockout cell lysate ab287353). To generate this image, wild-type and ATG7 knockout HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-ATG7 antibody [EP1759Y] (<a href='/en-us/products/primary-antibodies/atg7-antibody-ep1759y-ab52472'>ab52472</a>) at 1/100000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
ATG7 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human ATG7 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-atg7-knockout-hela-cell-line-ab283307'>ab283307</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
Jurkat cell lysate at 20 µg
Predicted band size: 77 kDa
Observed band size: 75 kDa
false
- Sanger seq
Lab
Sanger Sequencing - Human ATG7 knockout HeLa cell lysate (AB287353)
41 bp deletion in exon 4
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATG7 plays a significant role in autophagy a cellular degradation pathway critical for cell survival under stress. ATG7 contributes to the formation of autophagosomes by facilitating conjugation of ATG8 family proteins including LC3 to phosphatidylethanolamine. It acts within complexes that regulate cellular energy balance and stress responses ensuring cells maintain their function and integrity. Knockdown of ATG7 can impair autophagic flux highlighting its importance in maintaining cellular processes.
Pathways
ATG7 is a central player in the autophagy pathway influencing cellular metabolism and turnover. It interacts closely with ATG5 and ATG12 in this pathway to form a conjugation system essential for autophagosome elongation. Additionally ATG7 is involved in the mTOR signaling pathway which regulates nutrient sensing and cellular growth. Interaction with proteins like mTOR allows ATG7 to integrate signals from nutrient availability and stress responses finely tuning the autophagy process.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB133528
RabMAb|Recombinant|KO Validated|20ul selling size
![Immunocytochemistry/ Immunofluorescence - Anti-ATG7 antibody [EPR6251] (AB133528)](https://content.abcam.com/products/images/atg7-antibody-epr6251-ab133528--immunocytochemistry-immunofluorescence-img21537.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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