Human ATP10A knockout A549 cell lysate
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ATP10A KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 4 bp deletion in exon2.
View Alternative Names
AT10A_HUMAN, ATP10A, ATP10C, ATPVA, ATPVC, ATPase Class V type 10C, ATPase class V type 10A, ATPase type IV phospholipid transporting (P type), Aminophospholipid translocase VA, KIAA0566, Phospholipid transporting ATPase VA, Probable phospholipid-transporting ATPase VA
- Sanger seq
Unknown
Sanger Sequencing - Human ATP10A knockout A549 cell lysate (AB263099)
Allele-1 : 4 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human ATP10A knockout A549 cell lysate (AB263099)
Allele-2 : 1 bp insertion in exon2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATP10A contributes to maintaining the asymmetrical distribution of phospholipids in the cell membrane which is essential for cell function and integrity. This protein does not function alone; it associates with CDC50 to form a heterodimer complex important for its lipid translocase activity. This activity helps maintain cellular homeostasis impacting processes such as apoptosis and membrane curvature necessary for cell signaling and vesicle formation.
Pathways
The functional role of ATP10A integrates into lipid metabolism and cellular signaling pathways. ATP10A significantly impacts the phospholipid translocation pathway which regulates membrane dynamics and is associated with apoptosis-related proteins like scramblases. Within these pathways ATP10A also interacts with PI3K/AKT a signaling pathway important for cellular growth survival and metabolism.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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