Human ATP2C1 knockout HEK-293T cell lysate
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ATP2C1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 4 and 1 bp insertion in exon 4.
View Alternative Names
1700121J11Rik, AT2C1_HUMAN, ATP-dependent Ca(2+) pump PMR1, ATP2C1A, ATPase 2C1, ATPase Ca(2+) sequestering, ATPase Ca++ transporting type 2C member 1, AW061228, BCPM, Calcium-transporting ATPase type 2C member 1, D930003G21Rik, HHD, HUSSY 28, KIAA1347, MGC58010, MGC93231, OTTHUMP00000216066, OTTHUMP00000216068, OTTHUMP00000216069, OTTHUMP00000216071, OTTHUMP00000216072, OTTHUMP00000216073, OTTHUMP00000216074, OTTHUMP00000216075, PMR1, PMR1, rat, homolog of, PMR1L, SPCA, SPCA1, Secretory pathway Ca(2+) ATPase 1, Secretory pathway Ca(2+)-transporting ATPase, Secretory pathway Ca2+/Mn2+ ATPase 1, hSPCA1
- Sanger seq
Unknown
Sanger Sequencing - Human ATP2C1 knockout HEK-293T cell lysate (AB258322)
Allele-1 : 1 bp deletion in exon 4
- Sanger seq
Unknown
Sanger Sequencing - Human ATP2C1 knockout HEK-293T cell lysate (AB258322)
Allele-2 : 1 bp insertion in exon 4
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The efficient functioning of ATP2C1 regulates calcium ion concentration within the Golgi impacting protein sorting and processing. This protein contributes to cellular signaling and is a part of a broader family of P-type ATPases. Although ATP2C1 does not form part of a complex its activity indirectly influences the function of other calcium-binding proteins through its ion transport role.
Pathways
ATP2C1 participates in key calcium homeostasis pathways ensuring cellular functions related to calcium-dependent processes. It plays a role in the intracellular signaling pathway that involves calcium-regulated gene expression. ATP2C1 is functionally linked to proteins such as STIM1 and Orai1 which are critical in the process of calcium reabsorption and signaling.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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