Human ATP5E knockout HeLa cell lysate

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ATP5E also known as ATP synthase epsilon subunit is a component of the ATP synthase enzyme complex specifically the F1 portion. This protein has an approximate molecular mass of 5.6 kDa. ATP5E is mainly present in the inner mitochondrial membrane where it plays a role in ATP production. The synthesis of ATP is a process important for cellular energy and ATP5E contributes by stabilizing the structure of the ATP synthase complex.

Biological function summary

ATP5E functions as an integral part of the ATP synthase complex which comprises multiple subunits. This complex is essential for oxidative phosphorylation a primary method through which cells produce ATP. ATP5E's role is structural helping to keep the F1 catalytic core properly aligned during the conversion of ADP and inorganic phosphate into ATP. Through this biological activity ATP5E supports energy metabolism necessary for the survival of cells.

Pathways

ATP5E participates in the oxidative phosphorylation pathway a critical process for cellular respiration. This pathway involves electron transport and proton pumping processes that generate the proton gradient ATP synthase exploits to synthesize ATP. Other proteins like ATP5C1 the gamma subunit are closely related to ATP5E in this pathway each contributing to the efficient production of ATP from ADP and inorganic phosphate.

Associated diseases and disorders

ATP5E has connections with mitochondrial disorders such as Leigh syndrome. This disease results from mutations affecting mitochondrial function leading to severe energy deficits in cells. Mutations in ATP5E can disrupt the ATP synthase complex’s function contributing to the pathology of these disorders. ATP5A1 another subunit of the ATP synthase complex also links with these diseases underlining the importance of all these components in maintaining mitochondrial integrity and function.