Human ATP5E knockout HeLa cell lysate
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ATP5F1E KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
View Alternative Names
ATP synthase H+ transporting mitochondrial F1 complex epsilon subunit, ATP synthase epsilon chain mitochondrial, ATP synthase subunit epsilon, ATP5E_HUMAN, ATPE, ATPase subunit epsilon, F(0)F(1) ATPase, H(+) transporting two sector ATPase, MGC104243, Mitochondrial ATP synthase epsilon chain, Mitochondrial ATPase, mitochondrial
- Sanger seq
Unknown
Sanger Sequencing - Human ATP5E knockout HeLa cell lysate (AB263102)
Homozygous : 1 bp insertion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATP5E functions as an integral part of the ATP synthase complex which comprises multiple subunits. This complex is essential for oxidative phosphorylation a primary method through which cells produce ATP. ATP5E's role is structural helping to keep the F1 catalytic core properly aligned during the conversion of ADP and inorganic phosphate into ATP. Through this biological activity ATP5E supports energy metabolism necessary for the survival of cells.
Pathways
ATP5E participates in the oxidative phosphorylation pathway a critical process for cellular respiration. This pathway involves electron transport and proton pumping processes that generate the proton gradient ATP synthase exploits to synthesize ATP. Other proteins like ATP5C1 the gamma subunit are closely related to ATP5E in this pathway each contributing to the efficient production of ATP from ADP and inorganic phosphate.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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