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AB258789

Human ATP5J2 knockout HEK-293T cell lysate

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ATP5MF KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp insertion in exon 1.
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Sanger Sequencing - Human ATP5J2 knockout HEK-293T cell lysate (AB258789)
  • Sanger seq

Unknown

Sanger Sequencing - Human ATP5J2 knockout HEK-293T cell lysate (AB258789)

Homozygous : 4 bp insertion in exon 1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 4 bp insertion in exon 1.

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Complementary Products

Primary Antibodies

AB200715

Anti-ATP5J2 antibody

Western blot - Anti-ATP5J2 antibody (AB200715)

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Cell Lines & Lysates

AB266582

Human ATP5J2 knockout HEK-293T cell line

Sanger Sequencing - Human ATP5J2 knockout HEK-293T cell line (AB266582)

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Proteins & Peptides

AB314613

Recombinant Human ATP5J2 Protein (His Tag)

SDS-PAGE - Recombinant Human ATP5J2 Protein (His Tag) (AB314613)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
ATP5MF
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'ATP5J2' also known as ATP synthase-coupling factor 6 mitochondrial is a protein involved in the synthesis of ATP the primary energy carrier in cells. This protein integrates into the ATP synthase complex specifically the F0 part where it helps regulate proton flow. ATP5J2 is approximately 11 kDa in mass and appears predominantly in mitochondria the powerhouse of cells. It expresses widely in numerous tissues highlighting an important role in cellular energy metabolism fundamental to many physiological processes.
Biological function summary

ATP5J2 functions as an accessory subunit within the ATP synthase complex which is responsible for ATP production during oxidative phosphorylation. By participating in the regulation of proton translocation through the mitochondrial membrane ATP5J2 helps maintain the proton motive force necessary for ATP generation. The protein's involvement in metabolic energy conversion underlines its role in effective cellular respiration.

Pathways

ATP5J2 aligns with the oxidative phosphorylation pathway an essential metabolic route for energy production. It associates with other subunits of the ATP synthase complex such as ATP5B and ATP5O to fulfill regulatory roles in ATP synthesis. Additionally ATP5J2 has interactions within the mitochondrial electron transport chain contributing to the efficient function of this pathway and sustaining the energy requirements of cells.

Defects in ATP5J2 impact mitochondrial function linking it to mitochondrial diseases which often present with muscle weakness and neural disorders. Moreover abnormalities in ATP5J2 may associate with metabolic syndromes affecting global energy metabolism. These conditions highlight potential interactions with proteins like NDUFS1 another component of the electron transport chain where disturbances can exacerbate the impact of energy deficiencies on cellular health.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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