Human ATR knockout (hetero) A549 cell lysate
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ATR KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Heterozygous: (+/-): 49 bp deletion in exon 3.
View Alternative Names
ATR_HUMAN, Ataxia telangiectasia and Rad3 related, Ataxia telangiectasia and Rad3-related protein, FCTCS, FRAP-related protein 1, FRP-1, MEC1, MEC1 mitosis entry checkpoint 1 homolog, Protein kinase ATR, Rad3 related protein, SCKL, SCKL1, Serine/threonine-protein kinase ATR
- WB
Lab
Western blot - Human ATR knockout (hetero) A549 cell lysate (AB277987)
Lane 1 : Wild-type A549 cell lysate 20 ug
Lane 2 : ATR heterozygous knockout A549 cell lysate 20 ug
Lane 3 : HeLa cell lysate 20 ug
Western blot : Anti-ATR antibody staining at 1/15000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red.
In Western blot, ab10312 was shown to bind specifically to ATR. A band was observed at 260 kDa in wild-type A549 cell lysates with a reduction in signal observed at this size in ATR heterozygous knockout cell line ab276104 (knockout cell lysate ab277987). To generate this image, wild-type and ATR heterozygous knockout A549 cell lysates were analysed.
Nitrocellulose membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again then imaged.
Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-ATR antibody (<a href='/en-us/products/primary-antibodies/atr-antibody-ab10312'>ab10312</a>) at 1/15000 dilution
Lane 1:
Wild-type A549 cell lysate at 20 µg
Lane 2:
ATR knockout A549 cell lysate at 20 µg
Lane 2:
Western blot - Human ATR knockout (hetero) A549 cell line (<a href='/en-us/products/cell-lines/human-atr-knockout-hetero-a549-cell-line-ab276104'>ab276104</a>)
Lane 3:
HeLa cell lysate at 20 µg
Predicted band size: 301 kDa
Observed band size: 260 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human ATR knockout (hetero) A549 cell lysate (AB277987)
Heterozygous (+/-) : 49 bp deletion in exon 3.
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATR plays an essential role in maintaining genomic stability. It is part of a larger protein complex that includes ATRIP (ATR-interacting protein) which helps in localizing ATR to sites of DNA damage. Once activated ATR phosphorylates various substrates including CHK1 a critical checkpoint kinase involved in cell cycle arrest during DNA repair processes. The ability of ATR to coordinate with these proteins helps cells manage DNA damage effectively and prevent genomic instability.
Pathways
ATR functions centrally in the DNA damage response and repair mechanisms particularly the ATR-Chk1 pathway. This pathway interacts closely with the ATM (Ataxia Telangiectasia Mutated) pathway which also responds to DNA damage but usually to double-strand breaks. ATR primarily acts in response to replication stress and its activation leads to the arrest of the cell cycle allowing DNA repair to occur. This cooperation between ATR and ATM highlights their complementary roles in safeguarding genomic integrity under stress.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB195189
Alexa Fluor® 647 Anti-gamma H2A.X (phospho S139) antibody [EP854(2)Y]
BOND RX™ Validated|RabMAb|Recombinant
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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