Human AXL knockout HeLa cell lysate

• WB

Lab

Western blot - Human AXL knockout HeLa cell lysate (AB257152)

Western blot : Anti-Axl antibody [EPR21107] (ab215205) staining at 1 : 1000 dilution, shown in green; Anti-GAPDH antibody [6C5] - Loading Control (ab8245) shown in red.

ab215205 was shown to bind specifically to Axl. A band was observed at 140 kDa in wild-type HeLa cell lysates with no signal observed at this size in Axl CRISPR-Cas9 edited cell line ab265392 (CRISPR-Cas9 edited cell lysate ab257152). The band observed in the CRISPR-Cas9 edited lysate lane below 90 kDa is likely to represent a truncated form of Axl. This has not been investigated further and the functional properties of the gene product have not been determined.

Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/10000 dilution.

All lanes:

Western blot - Anti-Axl antibody [EPR21107] (<a href='/en-us/products/primary-antibodies/axl-antibody-epr21107-ab215205'>ab215205</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

AXL knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human AXL knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-axl-knockout-hela-cell-line-ab265392'>ab265392</a>)

Lane 2:

Western blot - Human AXL knockout HeLa cell lysate (ab257152)

Lane 3:

NCI-H1299 cell lysate at 20 µg

Lane 4:

Jurkat cell lysate at 20 µg

Predicted band size: 98 kDa

Observed band size: 80140 kDa

false

• Sanger seq

Unknown

Sanger Sequencing - Human AXL knockout HeLa cell lysate (AB257152)

Homozygous : 1 bp insertion in exon 7.