Human AZI2 knockout HeLa cell lysate
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AZI2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon2 and 7 bp deletion in exon2.
View Alternative Names
5-azacytidine-induced protein 2, AA410145, AZ2, AZI2_HUMAN, MGC112644, NAP-1, NF-kappa-B-activating kinase-associated protein 1, Nak-associated protein 1, TILP, TILP(S)
- WB
Lab
Western blot - Human AZI2 knockout HeLa cell lysate (AB257852)
Lane 1 : Wild-type HeLa cell lysate 20 μg
Lane 2 : AZI2 CRISPR-Cas9 edited HeLa cell lysate 20 μg
Lane 3 : HEK-293 cell lysate 20 μg
Lane 4 : Daudi cell lysate 20 μg
False colour image of Western blot : Anti-AZI2 antibody [EPR14698] - C-terminal staining at 1/5000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab192253 was shown to bind specifically to AZI2. A band was observed at 45 kDa in wild-type HeLa cell lysates with no signal observed at this size in AZI2 CRISPR-Cas9 edited cell line ab265686 (CRISPR-Cas9 edited cell lysate ab257852). The band observed in the CRISPR-Cas9 edited lysate lane below 45 kDa is likely to represent a truncated form of AZI2. This has not been investigated further and the functional properties of the gene product have not been determined. To generate this image, wild-type and AZI2 CRISPR-Cas9 edited HeLa cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Western blot - Anti-AZI2 antibody [EPR14698] - C-terminal (<a href='/en-us/products/primary-antibodies/azi2-antibody-epr14698-c-terminal-ab192253'>ab192253</a>) at 1/5000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
AZI2 CRISPR-Cas9 edited HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human AZI2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-azi2-knockout-hela-cell-line-ab265686'>ab265686</a>)
Lane 3:
HEK-293 cell lysate at 20 µg
Lane 4:
Daudi cell lysate at 20 µg
Predicted band size: 45 kDa
Observed band size: 45 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
AZI2 plays an essential role in the immune system. It is part of a signaling complex that includes other proteins such as TANK and TRAF6. This complex contributes to the activation of NF-kB transcription factors. AZI2 is involved in cellular processes like inflammation and response to stress by regulating the activity of NF-kB an important transcription factor. This regulatory activity underlines AZI2's significance in maintaining immune system homeostasis.
Pathways
AZI2 is closely associated with the toll-like receptor (TLR) and TNF receptor signaling pathways. These pathways orchestrate immune cell responses to microbial infections and inflammation. In these pathways AZI2 interacts with proteins like IKK epsilon and TANK to mediate NF-kB activation. These interactions help transduce signals from membrane-bound receptors to the nucleus promoting the expression of immune response genes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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