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AB261702

Human B2M (beta 2 Microglobulin) knockout A-431 cell lysate

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B2M KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9; X = 23 bp deletion, 1 bp deletion, 20 bp deletion; Frameshift = 99.8%.

View Alternative Names

B2M, B2MG_HUMAN, Beta 2 microglobin, Beta 2 microglobulin, Beta 2 microglobulin precursor, Beta chain of MHC class I molecules, Beta chain of mhc class 1 proteins, Beta-2-microglobulin form pI 5.3, CDABP0092, Hdcma22p, IMD43

2 Images
Western blot - Human B2M (beta 2 Microglobulin) knockout A-431 cell lysate (AB261702)
  • WB

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Western blot - Human B2M (beta 2 Microglobulin) knockout A-431 cell lysate (AB261702)

Western blot : Anti-beta 2 Microglobulin antibody [EP2978Y] staining at 1/5000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A]  (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab75853 was shown to bind specifically to beta 2 Microglobulin. A band was observed at 12 kDa in Wild-type A431 and HEK-293T cell lysates with no signal observed at this size in B2M (beta 2 Microglobulin) knockout A-431 cell line ab261893 (knockout cell lysate ab261702) or B2M knockout HEK-293T cell line ab266828 (knockout cell lysate ab256845). To generate this image, wild-type and B2M knockout A431 and HEK-293T cell lysates were analysed. Nitrocellulose membranes were blocked in fluorescent western blot (TBS-based) blocking solution in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.

All lanes:

Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] (<a href='/en-us/products/primary-antibodies/beta-2-microglobulin-antibody-ep2978y-ab75853'>ab75853</a>) at 1/5000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

B2M knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-b2m-beta-2-microglobulin-knockout-hek-293t-cell-line-ab266828'>ab266828</a>)

Lane 2:

Western blot - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell lysate (<a href='/en-us/products/cell-lysates/human-b2m-beta-2-microglobulin-knockout-hek-293t-cell-lysate-ab256845'>ab256845</a>)

Lane 3:

Wild-type A431 cell lysate at 20 µg

Lane 4:

B2M knockout A431 cell lysate at 20 µg

Lane 4:

Western blot - Human B2M (beta 2 Microglobulin) knockout A-431 cell line (<a href='/en-us/products/cell-lines/human-b2m-beta-2-microglobulin-knockout-a-431-cell-line-ab261893'>ab261893</a>)

Lane 4:

Western blot - Human B2M (beta 2 Microglobulin) knockout A-431 cell lysate (ab261702)

Predicted band size: 14 kDa

Observed band size: 12 kDa

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Next Generation Sequencing - Human B2M (beta 2 Microglobulin) knockout A-431 cell lysate (AB261702)
  • NGS

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Next Generation Sequencing - Human B2M (beta 2 Microglobulin) knockout A-431 cell lysate (AB261702)

X = 23 bp deletion, 1 bp deletion, 20 bp deletion

Key facts

Cell type

A-431

Species or organism

Human

Tissue

Skin

Knockout validation

Next Generation Sequencing,Western blot

Mutation description

Knockout achieved by CRISPR/Cas9; X = 23 bp deletion, 1 bp deletion, 20 bp deletion; Frameshift = 99.8%

Disease

Epidermoid Carcinoma

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "NGS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
B2M
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Next Generation Sequencing, Western blot
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.
Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Cell Lines & Lysates

AB266828

Human B2M (beta 2 Microglobulin) knockout HEK-293T cell line

Advanced Validation

Western blot - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell line (AB266828)

  • 0
  • 0
Primary Antibodies

AB75853

Anti-beta 2 Microglobulin antibody [EP2978Y]

KO Validated|RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-beta 2 Microglobulin antibody [EP2978Y] (AB75853)

  • 4
  • 9
Cell Lines & Lysates

AB257203

Human CBS knockout HeLa cell lysate

Western blot - Human CBS knockout HeLa cell lysate (AB257203)

  • 0
  • 0
Proteins & Peptides

AB151631

Recombinant Human beta 2 Microglobulin protein

SDS-PAGE - Recombinant Human beta 2 Microglobulin protein (AB151631)

  • 0
  • 0

Product promise

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For full details, please see our Terms & Conditions

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