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B2M KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

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Images

Western blot - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell lysate (AB256845), expandable thumbnail
  • Western blot - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell lysate (AB256845), expandable thumbnail
  • Sanger Sequencing - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell lysate (AB256845), expandable thumbnail

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

Alternative names

What's included?

1 Kit
Components
Human B2M knockout HEK293T cell lysate
1 x 100 µg
Human wild-type HEK293T cell lysate
1 x 100 µg

Recommended products

B2M KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

Key facts

Cell type

HEK-293T

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp insertion in exon 1.

Concentration
Loading...

Properties

Gene name

B2M

Gene editing type

Knockout

Gene editing method

CRISPR technology

Knockout validation

Sanger Sequencing

Zygosity

Homozygous

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

Storage

Shipped at conditions

Ambient - Can Ship with Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta-2-Microglobulin (B2M) is a component of the class I major histocompatibility complex (MHC I) and plays an important role in presenting peptides to the immune system. B2M weighs approximately 11.8 kDa and is found abundantly in all nucleated cells. It has alternate names such as B2 microglobulin or beta-2-microglobulin. This protein is present in the cell membrane as a part of the MHC I which is important for immune surveillance. Additionally B2M is detectable in various biological fluids including serum and its levels can reflect physiological and pathological states.

Biological function summary

Beta-2-microglobulin is important for the stability and transport of MHC class I molecules to the cell surface. As part of the MHC class I complex B2M assists in binding peptides allowing immune cells to identify and target pathogen-infected cells. Without B2M the MHC class I molecules are not properly expressed on the cell surface disrupting immune recognition. In laboratory settings researchers often use anti-beta-2-microglobulin antibodies to investigate its role in MHC class I function.

Pathways

Beta-2-microglobulin interacts significantly with the immune system most notably in the antigen processing and presentation pathway. It works alongside proteins such as the heavy chain of MHC class I. B2M is important in the pathway that involves the transport of antigens to the endoplasmic reticulum where they are loaded onto MHC class I molecules for inspection by cytotoxic T cells. Another related pathway is the tapasin-mediated processing of antigen peptides highlighting the indispensable role of B2M in immune response regulation.

Associated diseases and disorders

Beta-2-microglobulin is associated with conditions such as beta-2-microglobulin amyloidosis and certain lymphoproliferative disorders. Elevated levels of B2M in serum serve as a marker for diseases like multiple myeloma where the protein level correlates with disease severity. B2M-related amyloidosis frequently occurs in patients undergoing long-term dialysis where amyloid deposits accumulate in tissues. Linking B2M to immune system dysfunction studies have shown interactions with other proteins including components of the immune system like HLA-A and HLA-B highlighting B2M's relevance in diagnosing and understanding these conditions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Western blot - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell lysate (ab256845), expandable thumbnail

    Western blot - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell lysate (ab256845)

    Western blot: Anti-beta 2 Microglobulin antibody [EP2978Y] staining at 1/5000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A]  (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853 was shown to bind specifically to beta 2 Microglobulin. A band was observed at 12 kDa in Wild-type A431 and HEK-293T cell lysates with no signal observed at this size in B2M (beta 2 Microglobulin) knockout A-431 cell line Human B2M (beta 2 Microglobulin) knockout A-431 cell line ab261893 (knockout cell lysate Human B2M (beta 2 Microglobulin) knockout A-431 cell lysate ab261702) or B2M knockout HEK-293T cell line Human B2M (beta 2 Microglobulin) knockout HEK-293T cell line ab266828 (knockout cell lysate ab256845). To generate this image, wild-type and B2M knockout A431 and HEK-293T cell lysates were analysed.
    Nitrocellulose membranes were blocked in fluorescent western blot (TBS-based) blocking solution in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed in TBS-T, incubated with secondary antibodies Goat anti-Rabbit 800CW and Goat anti-Mouse 680RD at 1/20000 dilution for 1 h at room temperature, washed again then imaged.

    All lanes: Western blot - Anti-beta 2 Microglobulin antibody [EP2978Y] (Anti-beta 2 Microglobulin antibody [EP2978Y] ab75853) at 1/5000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: B2M knockout HEK-293T cell lysate at 20 µg

    Lane 3: Wild-type A431 cell lysate at 20 µg

    Lane 4: B2M knockout A431 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Observed band size: 12 kDa

  • Western blot - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell lysate (ab256845), expandable thumbnail

    Western blot - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell lysate (ab256845)

    Lane 1: Wild-type HEK-293T cell lysate 20 μg
    Lane 2: B2M knockout HEK-293T cell lysate 20 μg
    Lane 3: Wild-type A431 cell lysate 20 μg
    Lane 4: B2M knockout A431 cell lysate 20 μg
    False colour image of Western blot: Anti-beta 2 Microglobulin antibody [EPR21752-214] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, Anti-beta 2 Microglobulin antibody [EPR21752-214] ab218230 was shown to bind specifically to beta 2 Microglobulin. A band was observed at 12 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in B2M knockout cell line Human B2M (beta 2 Microglobulin) knockout HEK-293T cell line ab266828 (knockout cell lysate ab256845). To generate this image, wild-type and B2M knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.

    All lanes: Western blot - Anti-beta 2 Microglobulin antibody [EPR21752-214] (Anti-beta 2 Microglobulin antibody [EPR21752-214] ab218230) at 1/1000 dilution

    Lane 1: Wild-type HEK-293T cell lysate at 20 µg

    Lane 2: B2M knockout HEK-293T cell lysate at 20 µg

    Lane 3: Wild-type A431 cell lysate at 20 µg

    Lane 4: B2M knockout A431 cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 14 kDa

    Observed band size: 12 kDa

  • Sanger Sequencing - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell lysate (ab256845), expandable thumbnail

    Sanger Sequencing - Human B2M (beta 2 Microglobulin) knockout HEK-293T cell lysate (ab256845)

    Homozygous: 1 bp insertion in exon 1

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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