Human B4GALT2 knockout HEK-293T cell lysate
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B4GALT2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon 2 and 20 bp deletion in exon 2.
View Alternative Names
4-GalTase 2, 4-galactosyltransferase, 4-galactosyltransferase 2, B4GT2_HUMAN, Beta 1,4 GalTase 2, Beta 1,4 galactosyltransferase 2, Beta N acetylglucosaminyl glycolipid beta 1,4 galactosyltransferase 2, Beta-1, Beta-N-acetylglucosaminyl-glycolipid beta-1, Beta4Gal-T2, Nal synthase, UDP Gal:beta GlcNAc beta 1,4 galactosyltransferase 2, UDP Gal:betaGlcNAc beta 1,4 galactosyltransferase polypeptide 2, UDP galactose:beta N acetylglucosamine beta 1,4 galactosyltransferase 2, UDP-Gal:beta-GlcNAc beta-1, UDP-galactose:beta-N-acetylglucosamine beta-1
- Sanger seq
Unknown
Sanger Sequencing - Human B4GALT2 knockout HEK-293T cell lysate (AB263108)
Allele-1 : 20 bp deletion in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human B4GALT2 knockout HEK-293T cell lysate (AB263108)
Allele-2 : 1 bp deletion in exon 2
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The enzyme plays a role in critical cellular functions by participating in cell surface glycan formation. It is part of the galactosyltransferase family and works as a single polypeptide chain. B4GALT2 adds galactose residues in a beta 14 linkage on glycoproteins and glycolipids in the Golgi apparatus. This modification helps in cell communication and molecule stability.
Pathways
The B4GALT2 enzyme functions in the glycosylation pathway especially in the N-linked glycosylation process. It interacts with other enzymes like B4GALT1 and B4GALT3 involved in the same pathway. Both perform similar roles but work on different substrates or in different contexts in the cell contributing to the diversity of glycan structures seen in organisms.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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