Human BAG2 knockout HeLa cell lysate
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BAG2 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 2 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
BAG-family molecular chaperone regulator-2, BAG2_HUMAN, Bcl-2-associated athanogene 2, KIAA0576, MGC149462, OTTHUMP00000016668, dJ417I1.2, dJ417I1.2 (BAG family molecular chaperone regulator 2)
- WB
Lab
Western blot - Human BAG2 knockout HeLa cell lysate (AB257369)
Lane 1 : Wild-type HeLa cell lysate (20 ug)
Lane 2 : BAG2 knockout HeLa cell lysate (20 ug)
Lane 3 : HepG2 cell lysate (20 ug)
ab79406 was shown to specifically react with BAG2 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265907 (knockout cell lysate ab257369) was used. Wild-type and BAG2 knockout samples were subjected to SDS-PAGE. ab79406 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4oC at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-BAG2 antibody [EPR3567] (<a href='/en-us/products/primary-antibodies/bag2-antibody-epr3567-ab79406'>ab79406</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
BAG2 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human BAG2 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-bag2-knockout-hela-cell-line-ab265907'>ab265907</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 23 kDa
Observed band size: 25 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human BAG2 knockout HeLa cell lysate (AB257369)
Allele-1 : 2 bp insertion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human BAG2 knockout HeLa cell lysate (AB257369)
Allele-2 : Insertion of the selection cassette in exon 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The role of BAG2 involves maintaining protein homeostasis a critical aspect of cellular functions. It forms a complex with Hsp70 assisting in the proper folding of newly synthesized proteins and preventing their aggregation. BAG2 also impacts the ubiquitin-proteasome system by blocking the association of Hsp70 with CHIP an E3 ubiquitin ligase. This regulation prevents the proteolytic degradation of client proteins.
Pathways
BAG2 functions within the cellular stress response and protein folding pathways. It has a significant involvement in the Hsp70-mediated protein folding pathway where it influences the fate of various substrate proteins. The interaction of BAG2 with Hsp70 and the ubiquitin-proteasome pathway highlights its contribution to cellular homeostasis. Additionally BAG2's modulation of these pathways suggests a link with apoptosis regulation where it associates indirectly with Bcl-2 family members.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
![Western blot - Anti-BAG2 antibody [EPR3567] (AB79406)](https://content.abcam.com/products/images/bag2-antibody-epr3567-ab79406--western-blot-img121967.jpg)
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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