Human BAK1 (Bak) knockout HeLa cell lysate
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BAK1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 2 bp deletion in exon2 and Insertion of the selection cassette in exon2.
View Alternative Names
Apoptosis regulator BAK, BAK like, BAK_HUMAN, BCL2 antagonist/killer 1, Bak NT, Bcl 2 like 7 protein, Bcl-2 homologous antagonist/killer, Bcl-2-like protein 7, Bcl2-L-7, CDN1, Cell death inhibitor 1, MGC117255, MGC3887, NBak, Pro apoptotic protein BAK
- WB
Lab
Western blot - Human BAK1 (Bak) knockout HeLa cell lysate (AB257077)
Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : BAK1 knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab92999 observed at 23 kDa. Red - loading control ab8245 observed at 37 kDa.
ab92999 Anti-Bak antibody was shown to specifically react with Bak in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265277 (knockout cell lysate ab257077) was used. Wild-type and Bak knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab92999 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Bak antibody (<a href='/en-us/products/primary-antibodies/bak-antibody-ab92999'>ab92999</a>) at 1 µg/mL
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
BAK1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human BAK1 (Bak) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-bak1-bak-knockout-hela-cell-line-ab265277'>ab265277</a>)
Predicted band size: 23 kDa
Observed band size: 23 kDa
false
- WB
Lab
Western blot - Human BAK1 (Bak) knockout HeLa cell lysate (AB257077)
Lane 1 : Wild-type HeLa cell lysate (20μg)
Lane 2 : BAK1 knockout HeLa cell lysate (20μg)
Lanes 1- 2 : Merged signal (red and green). Green - ab32371 observed at 23 kDa. Red - loading control ab8245 observed at 37 kDa.
ab32371 Anti-Bak antibody [Y164] was shown to specifically react with Bak in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265277 (knockout cell lysate ab257077) was used. Wild-type and Bak knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab32371 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Bak antibody [Y164] (<a href='/en-us/products/primary-antibodies/bak-antibody-y164-ab32371'>ab32371</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
BAK1 knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human BAK1 (Bak) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-bak1-bak-knockout-hela-cell-line-ab265277'>ab265277</a>)
Predicted band size: 23 kDa
Observed band size: 23 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human BAK1 (Bak) knockout HeLa cell lysate (AB257077)
Allele-1 : 2 bp deletion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human BAK1 (Bak) knockout HeLa cell lysate (AB257077)
Allele-3 : Insertion of the selection cassette in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human BAK1 (Bak) knockout HeLa cell lysate (AB257077)
Allele-2 : 1 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Bak engages in promoting apoptosis by disrupting the mitochondrial outer membrane potential. It is part of the Bcl-2 family complex which balances cell survival and cell death. Bak collaborates with Bax protein to form pores in the mitochondrial membrane releasing cytochrome c and other apoptotic factors. This process initiates the cascade of caspases leading to programmed cell death.
Pathways
Bak is a critical component of the intrinsic apoptotic pathway. It directly interacts with pro-apoptotic proteins like Bax and anti-apoptotic proteins such as Bcl-2 and Bcl-xL. Bak's action in the apoptotic pathway involves a delicate balance between survival and death signals affecting cellular homeostasis. In addition Bak's interaction with the p53 pathway emphasizes its role in response to DNA damage ensuring damaged cells do not proliferate uncontrollably.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB32371
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bak antibody [Y164] (AB32371)](https://content.abcam.com/products/images/bak-antibody-y164-ab32371--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img422690.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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