Human BATF2 (SARI) knockout A549 cell lysate
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BATF2 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 4 bp deletion in exon3.
View Alternative Names
Basic leucine zipper transcriptional factor ATF like 2, MGC20410, Suppressor of AP 1 regulated by IFN
- Sanger seq
Unknown
Sanger Sequencing - Human BATF2 (SARI) knockout A549 cell lysate (AB257854)
Allele-1 : 4 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human BATF2 (SARI) knockout A549 cell lysate (AB257854)
Allele-2 : 1 bp insertion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
SARI modulates transcription processes involved in immune responses. It forms part of a regulatory complex that includes other proteins like TRAF3 and TBK1 to influence NF-κB signaling pathways. Through this complex SARI can suppress certain transcription factors thereby regulating gene expression linked to inflammation. This regulation helps maintain balanced immune responses ensuring cells do not overreact to stimuli.
Pathways
SARI interacts with the tumor necrosis factor (TNF) and toll-like receptor (TLR) signaling pathways. In these pathways SARI influences how cells respond to cytokines and infectious agents. It achieves this by interacting with key pathway proteins such as TRAF3 and RIPK1. These interactions allow SARI to mediate the downstream effects ultimately affecting cell survival and immune reactions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Male
Complementary Products
Primary Antibodies
AB219800
Anti-GSDMD antibody [EPR20859]
BOND RX™ Validated|RabMAb|Recombinant|KO Validated
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- 5
- 2
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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