BATF2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 2 bp deletion in exon3.
Images
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 2 bp deletion in exon3.
Alternative names
Basic leucine zipper transcriptional factor ATF like 2, MGC20410, Suppressor of AP 1 regulated by IFN
What's included?
Recommended products
BATF2 KO cell lysate available now. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 2 bp deletion in exon3.
Key facts
- Cell type
- A549
- Species or organism
- Human
- Tissue
- Lung
- Knockout validation
- Sanger Sequencing
- Mutation description
- Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and 2 bp deletion in exon3.
- Disease
- Carcinoma
- Concentration
Properties
- Gene name
- BATF2
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 1 US: 1
- Adherent/suspension
- Adherent
- Gender
- Male
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
SARI also known as suppressor of AP-1 regulates apoptosis and inflammation in cells. It is a protein with a molecular mass of around 64 kDa. SARI is mainly expressed in immune cells including macrophages and dendritic cells and also found in epithelial tissues. The protein acts mainly in the cytoplasm where it interacts with other molecules to carry out its functions.
- Biological function summary
SARI modulates transcription processes involved in immune responses. It forms part of a regulatory complex that includes other proteins like TRAF3 and TBK1 to influence NF-κB signaling pathways. Through this complex SARI can suppress certain transcription factors thereby regulating gene expression linked to inflammation. This regulation helps maintain balanced immune responses ensuring cells do not overreact to stimuli.
- Pathways
SARI interacts with the tumor necrosis factor (TNF) and toll-like receptor (TLR) signaling pathways. In these pathways SARI influences how cells respond to cytokines and infectious agents. It achieves this by interacting with key pathway proteins such as TRAF3 and RIPK1. These interactions allow SARI to mediate the downstream effects ultimately affecting cell survival and immune reactions.
- Associated diseases and disorders
SARI has roles in inflammatory diseases and cancer. In inflammatory diseases such as rheumatoid arthritis SARI influences the activity of inflammation-related proteins like NF-κB which can exacerbate or alleviate symptoms. In cancer SARI has been implicated in regulating tumor growth and metastasis interacting with other cancer-related proteins such as STAT3. These connections make SARI a potential therapeutic target in managing these conditions.
Product promise
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2 product images
Sanger Sequencing - Human BATF2 (SARI) knockout A549 cell lysate (ab257855)
Allele-2: 2 bp deletion in exon3
Sanger Sequencing - Human BATF2 (SARI) knockout A549 cell lysate (ab257855)
Allele-1: 1 bp insertion in exon3
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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