Human BAZ1A (ACF1) knockout HeLa cell lysate
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BAZ1A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 2.
View Alternative Names
ACF1, drosophila, homolog of, ATP dependent chromatin remodelling protein, ATP-dependent chromatin-remodeling protein, ATP-utilizing chromatin assembly and remodeling factor 1, Acf1, B930060C03, BAZ1A_HUMAN, BC065123, Bromodomain adjacent to zinc finger domain 1A, Bromodomain adjacent to zinc finger domain protein 1A, CHRAC subunit ACF1, DKFZp586E0518, FLJ14383, Gtl5, WALp1, WCRF180, Williams syndrome transcription factor-related chromatin-remodeling factor 180, cbp146, hACF1, hWALp1
- WB
Lab
Western blot - Human BAZ1A (ACF1) knockout HeLa cell lysate (AB257856)
Lane 1 : Wild-type HeLa cell lysate (20 μg)
Lane 2 : BAZIA knockout HeLa cell lysate (20 μg)
Lane 3 : Jurkat cell lysate (20 μg)
Lane 4 : Daudi cell lysate (20 μg)
Lanes 1-4 : Merged signal (red and green). Green - ab187670 observed at 178 kDa. Red - loading control ab7291 observed at 50 kDa.
ab187670 Anti-BAZ1A antibody [EPR15826] - C-terminal was shown to specifically react with BAZ1A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264925 (knockout cell lysate ab257856) was used. Wild-type and BAZ1A knockout samples were subjected to SDS-PAGE. ab187670 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (<a href='/en-us/products/primary-antibodies/acf1-baz1a-antibody-epr15826-c-terminal-ab187670'>ab187670</a>) at 1/10000 dilution
Lane 1:
Wild-type HeLa cell lysate at 20 µg
Lane 2:
BAZ1A knockout HeLa cell lysate at 20 µg
Lane 2:
Western blot - Human BAZ1A (ACF1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-baz1a-acf1-knockout-hela-cell-line-ab264925'>ab264925</a>)
Lane 3:
Jurkat cell lysate at 20 µg
Lane 4:
Daudi cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/20000 dilution
Predicted band size: 178 kDa
Observed band size: 178 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human BAZ1A (ACF1) knockout HeLa cell lysate (AB257856)
Homozygous : 11 bp deletion in exon 2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ACF1/BAZ1A is integral to chromatin remodeling which affects how DNA is packaged and accessed by other cellular machinery. It forms part of the ACF complex alongside the ISWI ATPase subunit heavily impacting nucleosome spacing and accessibility. ACF1/BAZ1A assists in nucleosome assembly and the establishment of regular chromatin structures important for gene expression regulation DNA replication and repair processes.
Pathways
ACF1/BAZ1A plays essential roles in pathways related to DNA repair and transcription regulation. In the DNA damage response pathway ACF1/BAZ1A works alongside other proteins like ISWI to ensure proper chromatin architecture facilitating DNA synthesis and repair mechanisms. It also contributes to transcription regulation by altering chromatin structure to allow transcription factors and RNA polymerase II access to DNA.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Primary Antibodies
AB187670
Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal
RabMAb|Recombinant|KO Validated
![Western blot - Anti-ACF1 / BAZ1A antibody [EPR15826] - C-terminal (AB187670)](https://content.abcam.com/products/images/acf1-baz1a-antibody-epr15826-c-terminal-ab187670--western-blot-img82160.jpg)
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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