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BAZ1A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 2.

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Images

Western blot - Human BAZ1A (ACF1) knockout HeLa cell lysate (AB257856), expandable thumbnail
  • Sanger Sequencing - Human BAZ1A (ACF1) knockout HeLa cell lysate (AB257856), expandable thumbnail

Key facts

Cell type
HeLa
Species or organism
Human
Tissue
Cervix
Knockout validation
Sanger Sequencing, Western blot
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 2.

Alternative names

ACF1, drosophila, homolog of, ATP dependent chromatin remodelling protein, ATP-dependent chromatin-remodeling protein, ATP-utilizing chromatin assembly and remodeling factor 1, Acf1, B930060C03, BAZ1A_HUMAN, BC065123, Bromodomain adjacent to zinc finger domain 1A, Bromodomain adjacent to zinc finger domain protein 1A, CHRAC subunit ACF1, DKFZp586E0518, FLJ14383, Gtl5, WALp1, WCRF180, Williams syndrome transcription factor-related chromatin-remodeling factor 180, cbp146, hACF1, hWALp1

What's included?

1 Kit
Components
Human BAZ1A knockout HeLa cell lysate
1 x 100 µg
Human wild-type HeLa cell lysate
1 x 100 µg

Recommended products

BAZ1A KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 2.

Key facts

Cell type
HeLa
Mutation description
Knockout achieved by using CRISPR/Cas9, Homozygous: 11 bp deletion in exon 2.
Disease
Adenocarcinoma
Concentration
Loading...

Properties

Gene name
BAZ1A
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous

Quality control

STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

Cell culture

Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female

Storage

Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C

Notes


Knockout cell lysate achieved by CRISPR/Cas9.

Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ACF1 also known as BAZ1A is a protein involved in DNA regulation. It is part of the ATP-utilizing chromatin assembly and remodeling factor (ACF) complex. This protein with a molecular mass of around 216 kDa is present mostly in the nucleus. ACF1 shows expression in various tissues including the brain and liver where it operates in regulating chromatin structure and gene accessibility.

Biological function summary

ACF1/BAZ1A is integral to chromatin remodeling which affects how DNA is packaged and accessed by other cellular machinery. It forms part of the ACF complex alongside the ISWI ATPase subunit heavily impacting nucleosome spacing and accessibility. ACF1/BAZ1A assists in nucleosome assembly and the establishment of regular chromatin structures important for gene expression regulation DNA replication and repair processes.

Pathways

ACF1/BAZ1A plays essential roles in pathways related to DNA repair and transcription regulation. In the DNA damage response pathway ACF1/BAZ1A works alongside other proteins like ISWI to ensure proper chromatin architecture facilitating DNA synthesis and repair mechanisms. It also contributes to transcription regulation by altering chromatin structure to allow transcription factors and RNA polymerase II access to DNA.

Associated diseases and disorders

ACF1/BAZ1A associates with conditions involving genomic instability and cancer. Its role in chromatin remodeling makes it relevant in cancer where faulty chromatin regulation can lead to unchecked cell proliferation. ACF1/BAZ1A has connections with p53 a protein significant in the cellular response to DNA damage further implicating its importance in maintaining genomic integrity. Understanding this protein's role could aid therapeutic strategies targeting chromatin dynamics in these diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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