BAZ1B KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.
Images
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.
Alternative names
BAZ1B_HUMAN, Bromodomain adjacent to zinc finger domain protein 1B, Tyrosine-protein kinase BAZ1B, WALP-2, WBRS-9, WBSC-10, WBSCR10, WBSCR9, Williams Beuren syndrome chromosome region 9 protein, Williams syndrome transcription factor, Williams-Beuren syndrome chromosomal region 10 protein, Williams-Beuren syndrome chromosomal region 9 protein, hWALP-2, transcription factor WSTF
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BAZ1B KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.
Key facts
- Cell type
- HeLa
- Species or organism
- Human
- Tissue
- Cervix
- Knockout validation
- Sanger Sequencing, Western blot
- Mutation description
- Knockout achieved by using CRISPR/Cas9, Homozygous: 5 bp deletion in exon 2.
- Disease
- Adenocarcinoma
- Concentration
Properties
- Gene name
- BAZ1B
- Gene editing type
- Knockout
- Gene editing method
- CRISPR technology
- Knockout validation
- Sanger Sequencing, Western blot
- Zygosity
- Homozygous
Quality control
- STR analysis
- CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
- Biosafety level
- EU: 2 US: 2
- Adherent/suspension
- Adherent
- Gender
- Female
Storage
- Shipped at conditions
- Ambient - Can Ship with Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
Notes
Knockout cell lysate achieved by CRISPR/Cas9.
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
WSTF also known as Williams Syndrome Transcription Factor or BAZ1B is a versatile protein with an estimated mass around 175 kDa. It is a component of the nucleosome remodeling factor (NURF) and the Williams-Beuren syndrome chromosome region 17 (WBSCR17) complex. WSTF has widespread expression in various tissues including the brain and heart which highlights its involvement in diverse cellular processes. Functionally it facilitates chromatin remodeling by altering the structure of nucleosomes which is vital for DNA accessibility in transcription DNA repair and replication.
- Biological function summary
Since WSTF participates in chromatin structure modulation it plays a considerable role in transcriptional regulation. It is a part of the NURF complex which makes it essential for DNA accessibility and gene expression. The complex interacts with other proteins to allow chromatin to accommodate active transcription by repositioning nucleosomes. WSTF also has a kinase domain that phosphorylates histone H2A. This phosphorylation integrates signals that coordinate transcription and DNA damage repair mechanisms highlighting its multifunctional nature in maintaining genomic stability.
- Pathways
WSTF has pivotal roles in the chromatin remodeling and DNA repair pathways. The chromatin remodeling pathway involves WSTF's interaction with the transcription factor complex influencing gene accessibility and expression. WSTF in the DNA repair pathway ensures proper genomic integrity through its association with proteins like BRCA1 highlighting its participation in the cellular response to DNA damage. By engaging with these pathways WSTF contributes to the cell's ability to regulate the genome and respond to damage efficiently.
- Associated diseases and disorders
WSTF is significantly connected to Williams-Beuren syndrome and certain cancers. Williams-Beuren syndrome a developmental disorder arises from the deletion of the region on chromosome 7 involving the WSTF gene affecting brain and heart function. In certain cancers aberrant expression or mutations within the WSTF gene are associated with uncontrolled chromatin remodeling contributing to tumorigenesis. Through these disorders WSTF shows its interaction with proteins like SMARCA1 in chromatin remodeling anomalies emphasizing its importance in disease progression and cellular dysfunction.
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3 product images
Western blot - Human BAZ1B (WSTF) knockout HeLa cell lysate (ab257370)
Lane 1: Wild-type HeLa cell lysate (20μg)
Lane 2: BAZ1B knockout HeLa cell lysate (20μg)
Lanes 1- 2: Merged signal (red and green). Green - Anti-WSTF antibody [EPR1703] ab109439 observed at 171 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.
Anti-WSTF antibody [EPR1703] ab109439 Anti-WSTF antibody [EPR1703] was shown to specifically react with WSTF in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human BAZ1B (WSTF) knockout HeLa cell line ab264907 (knockout cell lysate ab257370) was used. Wild-type and WSTF knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-WSTF antibody [EPR1703] ab109439 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-WSTF antibody [EPR1703] (Anti-WSTF antibody [EPR1703] ab109439) at 1/1000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: WSTF knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human BAZ1B (WSTF) knockout HeLa cell line (Human BAZ1B (WSTF) knockout HeLa cell line ab264907)
Performed under reducing conditions.
Predicted band size: 171 kDa
Observed band size: 171 kDa
Western blot - Human BAZ1B (WSTF) knockout HeLa cell lysate (ab257370)
Lane 1: Wild-type HeLa cell lysate (20μg)
Lane 2: BAZ1B knockout HeLa cell lysate (20μg)
Lanes 1- 2: Merged signal (red and green). Green - Anti-WSTF antibody [EP1704Y] ab51256 observed at 175 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.
Anti-WSTF antibody [EP1704Y] ab51256 Anti-WSTF antibody [EP1704Y] was shown to specifically react with WSTF in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line Human BAZ1B (WSTF) knockout HeLa cell line ab264907 (knockout cell lysate ab257370) was used. Wild-type and WSTF knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. Anti-WSTF antibody [EP1704Y] ab51256 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 15000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.All lanes: Western blot - Anti-WSTF antibody [EP1704Y] (Anti-WSTF antibody [EP1704Y] ab51256) at 1/15000 dilution
Lane 1: Wild-type HeLa cell lysate at 20 µg
Lane 2: BAZ1B knockout HeLa cell lysate at 20 µg
Lane 2: Western blot - Human BAZ1B (WSTF) knockout HeLa cell line (Human BAZ1B (WSTF) knockout HeLa cell line ab264907)
Performed under reducing conditions.
Predicted band size: 171 kDa
Observed band size: 175 kDa
Sanger Sequencing - Human BAZ1B (WSTF) knockout HeLa cell lysate (ab257370)
Homozygous: 5 bp deletion in exon 2
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