Human BCAR3 (NSP2) knockout HeLa cell lysate
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BCAR3 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon3 and 23 bp deletion in exon3 and 29 bp deletion in exon3.
View Alternative Names
BCAR3_HUMAN, Breast cancer anti estrogen resistance 3, Breast cancer anti-estrogen resistance protein 3, KIAA0554, NSP 2, Novel SH2-containing protein 2, SH2 containing protein Nsp2, SH2 domain-containing protein 3B, SH2D3B, dJ1033H22.2, dJ1033H22.2 breast cancer anti estrogen resistance 3
- Sanger seq
Unknown
Sanger Sequencing - Human BCAR3 (NSP2) knockout HeLa cell lysate (AB263109)
Allele-3 : 10 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human BCAR3 (NSP2) knockout HeLa cell lysate (AB263109)
Allele-2 : 23 bp deletion in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human BCAR3 (NSP2) knockout HeLa cell lysate (AB263109)
Allele-1 : 29 bp deletion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com