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AB257858

Human BCR knockout HEK-293T cell lysate

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BCR KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 2 bp insertion in exon1.

View Alternative Names

BCR/ABL FUSION GENE, INCLUDED, BCR/FGFR1 FUSION GENE, INCLUDED, BCR/FGFR1 chimera protein, BCR/PDGFRA FUSION GENE, INCLUDED, BCR1, BCR_HUMAN, Breakpoint cluster region, Breakpoint cluster region protein, CML, D22S11, D22S662, FGFR1/BCR chimera protein, Renal carcinoma antigen NY-REN-26

3 Images
Western blot - Human BCR knockout HEK-293T cell lysate (AB257858)
  • WB

Lab

Western blot - Human BCR knockout HEK-293T cell lysate (AB257858)

Lane 1 : Wild-type HEK-293T cell lysate 20 ug
Lane 2 : BCR knockout HEK-293T cell lysate 20 ug

Lanes 1 - 2 : Merged signal (red and green). Green - ab222406 observed at 150 kDa. Red - loading control, ab7291 (Mouse anti-Alpha Tubulin [DM1A]) observed at 55kDa.

ab222406 was shown to react with BCR in wild-type HEK-293T cells in western blot. The bands observed in BCR knockout cell line ab266583 (BCR knockout cell lysate ab257858) below 150kDa may represent truncated forms and cleaved fragments. This has not been investigated further. Wild-type and BCR knockout HEK-293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab222406 and ab7291 (Mouse anti-Alpha Tubulin [DM1A]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

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Sanger Sequencing - Human BCR knockout HEK-293T cell lysate (AB257858)
  • Sanger seq

Unknown

Sanger Sequencing - Human BCR knockout HEK-293T cell lysate (AB257858)

Allele-2 : 2 bp insertion in exon1

Sanger Sequencing - Human BCR knockout HEK-293T cell lysate (AB257858)
  • Sanger seq

Unknown

Sanger Sequencing - Human BCR knockout HEK-293T cell lysate (AB257858)

Allele-1 : 1 bp insertion in exon1

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 2 bp insertion in exon1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.</p>" } } }
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Product details

Western blot data indicates that the CRISPR gene edit may have resulted in a truncation of the protein of interest. Please see data images.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab257858-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human BCR knockout HEK293T cell lysate", "number":"AB257858-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB257858-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
BCR
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Bcr protein also known as breakpoint cluster region protein plays an important role in cellular signaling. It has a molecular mass of approximately 160 kDa and is expressed in many cell types such as hematopoietic cells. Bcr functions as a GTPase-activating protein (GAP) for p21rac and other small GTP-binding proteins. This activity influences cellular processes such as growth and differentiation. Bcr is also known for its involvement in the formation of the Bcr-Abl fusion protein an important factor in certain leukemias.
Biological function summary

The Bcr protein acts within cells to regulate signal transduction and cytoskeletal organization. It is part of the pre-B cell receptor (pre-BCR) signaling complex influencing B-cell development. Its GAP activity is important for the regulation of small GTPases impacting cell migration and adhesion. Alterations in Bcr function can affect cellular responses such as apoptosis making it a focus for research into targeted therapies for hematological conditions.

Pathways

The Bcr protein is an important player in signaling pathways involving the Ras superfamily of small GTPases. It is notably involved in the Rho GTPase pathway tied to cytoskeletal dynamics and cell cycle progression. Bcr's interaction with these pathways puts it in relation to proteins like Rac and Cdc42 which are vital for various cellular processes. Disruption in Bcr-associated pathways can lead to aberrant cell growth and metastasis.

Bcr has a well-documented link to certain leukemias particularly chronic myeloid leukemia (CML). The Bcr-Abl fusion protein resulting from the translocation of chromosomes 9 and 22 is central to CML pathology. Additionally Bcr connects with other proteins such as Abl in this disease context. Further disruptions in Bcr signaling can relate to disorders involving immune system dysregulation demonstrating the protein's relevance in both oncology and immunology research.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB187831

Anti-Bcr-abl antibody [7C6]

Recombinant

Immunocytochemistry/ Immunofluorescence - Anti-Bcr-abl antibody [7C6] (AB187831)

  • 0
  • 0
Proteins & Peptides

AB72493

Recombinant Human Bcr protein

SDS-PAGE - Recombinant Human Bcr protein (AB72493)

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Cell Lines & Lysates

AB266714

Human BCR knockout HEK-293T cell line

Advanced Validation

Sanger Sequencing - Human BCR knockout HEK-293T cell line (AB266714)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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