Human BECN1 (Beclin 1) knockout HeLa cell lysate
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BECN1 KO cell lysate available now. KO validated by Next Generation Sequencing, Western blot. Free of charge wild type control included. Knockout achieved by CRISPR/Cas9 X = 4 bp deletion Frameshift: 93.41%.
View Alternative Names
APG6, ATG 6, ATG6 autophagy related 6 homolog, BECN1_HUMAN, Bcl-2-interacting protein beclin, Beclin 1 (coiled coil moesin like BCL2 interacting protein), Beclin 1 autophagy related, Beclin-1, Coiled-coil myosin-like BCL2-interacting protein, GT197, Protein GT197, VPS 30
- WB
Lab
Western blot - Human BECN1 (Beclin 1) knockout HeLa cell lysate (AB263936)
Lane 1 : Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : BECN1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lanes 1 - 2 : Merged signal (red and green). Green - ab118148 observed at 60 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab118148 was shown to react with Beclin 1 in wild-type HeLa cells in western blot Loss of signal was observed when BECN1 knockout cell line ab262511 (knockout cell lysate ab263936) was used. Wild-type HeLa and BECN1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in non-mammalian (TBS-based) blocking solution before incubation with ab118148 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Beclin 1 antibody [OTI4A10] (<a href='/en-us/products/primary-antibodies/beclin-1-antibody-oti4a10-ab118148'>ab118148</a>) at 1/1000 dilution
Lane 1:
Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2:
BECN1 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 2:
Western blot - Human BECN1 (Beclin 1) knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-becn1-beclin-1-knockout-hela-cell-line-ab262511'>ab262511</a>)
Predicted band size: 52 kDa
Observed band size: 60 kDa
false
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein functions as an important component of the Beclin 1 autophagy complex which orchestrates the autophagic process. This complex is essential for promoting autophagosome nucleation by interacting with multiple partners that regulate the autophagy pathway. In addition to its role in autophagy Beclin 1 protein stabilizes interactions with other autophagic proteins ensuring the correct assembly and activity of this cellular mechanism. The regulation of Beclin 1's activity and expression influences the efficiency and specificity of autophagy in cells.
Pathways
Beclin 1 integrates into critical cellular pathways like the PI3K/Akt pathway and the mTOR pathway which are pivotal in cell growth and survival. Within these pathways beclin cooperates closely with proteins such as ATG14 and VPS34. These interactions facilitate the recruitment and activation of additional factors that promote the elongation of phagophores which eventually become complete autophagosomes. The coordination of these pathways ensures the proper balance between cell survival and death adapting to cellular conditions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Cell Lines & Lysates
AB262511
Human BECN1 (Beclin 1) knockout HeLa cell line
Advanced Validation
- 0
- 0
Multiplex Kits
AB228525
Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel
Recombinant
- 5
- 1
Primary Antibodies
AB211324
Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451]
KO Validated|RabMAb|Recombinant|Lab Essentials
![Western blot - Anti-SQSTM1 / p62 (phospho S349) antibody [EPR20451] (AB211324)](https://content.abcam.com/products/images/sqstm1-p62-phospho-s349-antibody-epr20451-ab211324--western-blot-img23347.jpg)
- 5
- 3
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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