Human BMI1 knockout HEK-293T cell lysate
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BMI1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 543 bp deletion in exon2.
View Alternative Names
Polycomb group RING finger protein 4, BMI1, RNF51, Polycomb complex protein BMI-1, RING finger protein 51, PCGF4
- WB
Lab
Western blot - Human BMI1 knockout HEK-293T cell lysate (AB256850)
Lane 1 : Wild-type HEK293T cell lysate (20 ug)
Lane 2 : BMI1 knockout HEK293T cell lysate (20 ug)
Lane 3 : MOLT-4 cell lysate (20 ug)
ab126783 was shown to specifically react with Bmi1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266514 (knockout cell lysate ab256850) was used. Wild-type and Bmi1 knockout samples were subjected to SDS-PAGE. ab126783 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated at room temperature for 2.5 hours at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Bmi1 antibody [EPR3745(2)] (<a href='/en-us/products/primary-antibodies/bmi1-antibody-epr37452-ab126783'>ab126783</a>) at 1/1000 dilution
Lane 1:
Wild-type HEK293T cell lysate at 20 µg
Lane 2:
BMI1 knockout HEK293T cell lysate at 20 µg
Lane 2:
Western blot - Human BMI1 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-bmi1-knockout-hek-293t-cell-line-ab266514'>ab266514</a>)
Lane 3:
MOLT-4 cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 37 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human BMI1 knockout HEK-293T cell lysate (AB256850)
Homozygous : 543 bp deletion in exon2
Complementary Products
Primary Antibodies
AB126783
Anti-Bmi1 antibody [EPR3745(2)]
20ul selling size|Recombinant|KO Validated|Advanced Validation|RabMAb
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Bmi1 antibody [EPR3745(2)] (AB126783)](https://content.abcam.com/products/images/bmi1-antibody-epr37452-ab126783--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img57523.jpg)
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- 3
Primary Antibodies
AB216347
Anti-SNAIL antibody [EPR21043]
20ul selling size|KO Validated|RabMAb|Recombinant
![Immunoprecipitation - Anti-SNAIL antibody [EPR21043] (AB216347)](https://content.abcam.com/products/images/snail-antibody-epr21043-ab216347--immunoprecipitation-img143315.jpg)
- 1
- 1
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The functions of Bmi1 extend into maintaining stem cell renewal and differentiation. It acts as part of the Polycomb repressive complex 1 (PRC1) partnering with other proteins to regulate gene expression by altering chromatin structure. Bmi1 protein regulates cell proliferation and inhibits senescence by repressing the INK4a/ARF locus which encodes tumor suppressor proteins like p16INK4a and p14ARF.
Pathways
Bmi1 plays significant roles in processes such as the DNA damage response and the self-renewal of hematopoietic stem cells. It is involved in the modulation of the Wnt and Notch signaling pathways. Bmi1 protein interacts closely with proteins such as CBX2 and RNF2 within these pathways affecting the transcriptional landscape that governs cell fate decisions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com