Human BMI1 knockout MCF7 cell lysate
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BMI1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon2 and 1 bp insertion in exon2.
View Alternative Names
B lymphoma Mo MLV insertion region (mouse), B lymphoma Mo MLV insertion region 1 homolog, BMI1 polycomb ring finger oncogene, BMI1_HUMAN, Flvi 2/bmi 1, MGC12685, Murine leukemia viral (bmi 1) oncogene homolog, Oncogene BMI 1, PCGF 4, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, Polycomb group protein Bmi1, Polycomb group ring finger 4, RING finger protein 51, RNF 51
- WB
Lab
Western blot - Human BMI1 knockout MCF7 cell lysate (AB256851)
Lane 1 : Wild-type MCF7 cell lysate (20µg)
Lane 2 : BMI1 knockout MCF7 cell lysate (20µg)
Lane 3 : A431 cell lysate (20µg)
Lane 4 : HEK-293 cell lysate (20µg)
Lanes 1- 4 : Merged signal (red and green). Green - ab269678 observed at 36 kDa. Red - loading control ab52866 observed at 50 kDa.
ab269678 Mouse monoclonal [BMI1/2823] to Bmi1 was shown to specifically react with Bmi1 in wild-type MCF7 cells in western blot. Loss of signal was observed when knockout cell line ab262319 (knockout cell lysate ab256851) was used. Wild-type and Bmi1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab269678 and Anti-alpha Tubulin antibody [EP1332Y] - Microtubule Marker (ab52866) were incubated overnight at 4°C at 2 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Bmi1 antibody [BMI1/2823] (<a href='/en-us/products/primary-antibodies/bmi1-antibody-bmi1-2823-ab269678'>ab269678</a>) at 2 µg/mL
Lane 1:
Wild-type MCF7 cell lysate at 20 µg
Lane 1:
Western blot - Human BMI1 knockout MCF7 cell lysate (ab256851)
Lane 2:
BMI1 knockout MCF7 cell lysate at 20 µg
Lane 2:
Western blot - Human BMI1 knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-bmi1-knockout-mcf7-cell-line-ab262319'>ab262319</a>)
Lane 3:
A431 cell lysate at 20 µg
Lane 4:
HEK-293 cell lysate at 20 µg
Predicted band size: 36 kDa
Observed band size: 36 kDa
false
- WB
Lab
Western blot - Human BMI1 knockout MCF7 cell lysate (AB256851)
Lane 1 : Wild-type MCF7 cell lysate (20µg)
Lane 2 : BMI1 knockout MCF7 cell lysate (20µg)
Lane 3 : A431 cell lysate (20µg)
Lanes 1- 3 : Merged signal (red and green). Green - ab126783 observed at 37 kDa. Red - loading control ab7291 observed at 50 kDa.
ab126783 Rabbit monoclonal [EPR3745(2)] to Bmi1 was shown to specifically react with Bmi1 in wild-type MCF7 cells in western blot. Loss of signal was observed when knockout cell line ab262319 (knockout cell lysate ab256851) was used. Wild-type and Bmi1 knockout samples were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab126783 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
All lanes:
Western blot - Anti-Bmi1 antibody [EPR3745(2)] (<a href='/en-us/products/primary-antibodies/bmi1-antibody-epr37452-ab126783'>ab126783</a>) at 1/10000 dilution
Lane 1:
Wild-type MCF7 cell lysate at 20 µg
Lane 2:
BMI1 knockout MCF7 cell lysate at 20 µg
Lane 2:
Western blot - Human BMI1 knockout MCF7 cell line (<a href='/en-us/products/cell-lines/human-bmi1-knockout-mcf7-cell-line-ab262319'>ab262319</a>)
Lane 3:
A431 cell lysate at 20 µg
Predicted band size: 171 kDa,36 kDa,43 kDa,67 kDa
Observed band size: 175 kDa,37 kDa,67 kDa
false
- Sanger seq
Unknown
Sanger Sequencing - Human BMI1 knockout MCF7 cell lysate (AB256851)
Allele-2 : 1 bp insertion in exon2
- Sanger seq
Unknown
Sanger Sequencing - Human BMI1 knockout MCF7 cell lysate (AB256851)
Allele-1 : 1 bp deletion in exon2
Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The functions of Bmi1 extend into maintaining stem cell renewal and differentiation. It acts as part of the Polycomb repressive complex 1 (PRC1) partnering with other proteins to regulate gene expression by altering chromatin structure. Bmi1 protein regulates cell proliferation and inhibits senescence by repressing the INK4a/ARF locus which encodes tumor suppressor proteins like p16INK4a and p14ARF.
Pathways
Bmi1 plays significant roles in processes such as the DNA damage response and the self-renewal of hematopoietic stem cells. It is involved in the modulation of the Wnt and Notch signaling pathways. Bmi1 protein interacts closely with proteins such as CBX2 and RNF2 within these pathways affecting the transcriptional landscape that governs cell fate decisions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 1 US: 1
Adherent/suspension
Adherent
Gender
Female
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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