Human BMP2 knockout HeLa cell lysate
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- WB
Supplier Data
Western blot - Human BMP2 knockout HeLa cell lysate (AB256852)
Blocking and diluting buffer and concentration : 5% NFDM/TBST.
Performed under reducing conditions.
In Western blot, ab325708 was shown to bind specifically to BMP2. Target of interest was observed at 35 kDa in HeLa cell lysates (lane 1) with no signal observed at this size in BMP2 knockout cell line (lane 2, knockout cell line ab261767 / knockout lysate ab256852).
The identity of the lower MW band at approximately 12 kDa is unknown.
In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
All lanes:
Western blot - Anti-BMP2 antibody [EPR30246-26] (<a href='/en-us/products/primary-antibodies/bmp2-antibody-epr30246-26-ab325708'>ab325708</a>) at 1/1000 dilution
Lane 1:
Western blot - Human BMP2 knockout HeLa cell lysate (ab256852) at 20 µg
Lane 2:
BMP2 knockout HeLa whole cell lysate at 20 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution
Observed band size: 35 kDa,36 kDa
false
Exposure time: 70s
- Sanger seq
Unknown
Sanger Sequencing - Human BMP2 knockout HeLa cell lysate (AB256852)
Allele-2 : Insertion of the selection cassette in exon 2
- Sanger seq
Unknown
Sanger Sequencing - Human BMP2 knockout HeLa cell lysate (AB256852)
Allele-1 : 17 bp deletion in exon 2
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Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com