Human BRD9 knockout HEK-293T cell lysate

• WB

Lab

Western blot - Human BRD9 knockout HEK-293T cell lysate (AB258336)

Lane 1 : Wild-type HEK-293T cell lysate 20 μg

Lane 2 : BRD9 knockout HEK-293T cell lysate 20 μg

False colour image of Western blot : Anti-BRD9 antibody [EPR23888-5] staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody [6C5] (ab8245) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab259839 was shown to bind specifically to BRD9. A band was observed at 75 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BRD9 knockout cell line ab266763 (knockout cell lysate ab258336). To generate this image, wild-type and BRD9 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 ^°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-BRD9 antibody [EPR23888-5] (<a href='/en-us/products/primary-antibodies/brd9-antibody-epr23888-5-ab259839'>ab259839</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

BRD9 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human BRD9 knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-brd9-knockout-hek-293t-cell-line-ab266763'>ab266763</a>)

Predicted band size: 67 kDa

Observed band size: 75 kDa

false

• Sanger seq

Unknown

Sanger Sequencing - Human BRD9 knockout HEK-293T cell lysate (AB258336)

Homozygous : 352 bp deletion in exon3