Human BRK1 (C3orf10) knockout HeLa cell lysate
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BRK1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1 and 4 bp deletion in exon1 and 88 bp insertion in exon1.
View Alternative Names
BRICK1, SCAR/WAVE actin nucleating complex subunit, BRICK1, SCAR/WAVE actin nucleating complex subunit, homolog, Brk1-like, C3ORF10 GENE, C3orf10 chromosome 3 open reading frame 10, Chromosome 3 open reading frame 10, HSPC300, Haematopoietic stem/progenitor cell protein 300, MDS027, Probable protein BRICK1, Protein BRICK1, hHBrk1
- Sanger seq
Unknown
Sanger Sequencing - Human BRK1 (C3orf10) knockout HeLa cell lysate (AB258794)
Allele-2 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human BRK1 (C3orf10) knockout HeLa cell lysate (AB258794)
Allele-3 : 88 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human BRK1 (C3orf10) knockout HeLa cell lysate (AB258794)
Allele-1 : 4 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
The protein product of C3orf10 may participate in cellular structural organization or intracellular signaling. Evidence suggests it functions as part of a larger protein complex though not extensively characterized. It possibly assists in linking cellular components to ensure structural integrity or facilitate signaling processes in cells.
Pathways
C3orf10 integrates into certain cellular pathways potentially involving cytoskeleton organization or signaling cascades. It associates with proteins involved in structural support and signal relay including some members of the armadillo family known for roles in cell adhesion and signaling. However the precise pathways C3orf10 participates in remain to be fully elucidated.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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