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AB258795

Human BRPF1 (Peregrin) knockout HEK-293T cell lysate

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BRPF1 KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 34 bp deletion in exon 2.

View Alternative Names

BR140, BRPF1_HUMAN, Bromodomain and PHD finger containing 1, Bromodomain and PHD finger-containing protein 1, Peregrin, Protein Br140, bromodomain-containing protein, 140kD

3 Images
Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell lysate (AB258795)
  • WB

Lab

Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell lysate (AB258795)

Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : BRPF1 knockout HEK-293T cell lysate 20 μg
Lane 3 : BRPF1 knockout HEK-293T cell lysate 20 μg
Lane 4 : Empty
Lane 5 : PC-3 cell lysate 20 μg
Lane 6 : HepG2 cell lysate 20 μg
False colour image of Western blot : Anti-Peregrin/BRPF1 antibody [EPR24069-57] staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab259840 was shown to bind specifically to Peregrin/BRPF1. A band was observed at 120 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BRPF1 knockout cell line ab266549 (knockout cell lysate ab258795). To generate this image, wild-type and BRPF1 knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

Lanes 1, 2, 3, 5 and 6:

Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (<a href='/en-us/products/primary-antibodies/peregrin-brpf1-antibody-epr24069-57-ab259840'>ab259840</a>) at 1/500 dilution

Lane 4:

Western blot - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (<a href='/en-us/products/primary-antibodies/peregrin-brpf1-antibody-epr24069-57-ab259840'>ab259840</a>)

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lanes 2 - 3:

BRPF1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-brpf1-peregrin-knockout-hek-293t-cell-line-ab266549'>ab266549</a>)

Lane 4:

Empty

Lane 5:

PC-3 cell lysate at 20 µg

Lane 6:

HepG2 cell lysate at 20 µg

Predicted band size: 137 kDa

Observed band size: 120 kDa

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Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell lysate (AB258795)
  • WB

Lab

Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell lysate (AB258795)

False colour image of Western blot : Anti-Peregrin/BRPF1 antibody - N-terminal staining at 1/500 dilution shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution shown in red. In Western blot ab226909 was shown to bind specifically to Peregrin/BRPF1. A band was observed at 160 145 and 138 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BRPF1 knockout cell line ab266549 (knockout cell lysate ab258795). To generate this image wild-type and BRPF1 knockout HEK-293T cell lysates were analysed. First samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °. Blots were washed four times in TBS-T incubated with secondary antibodies for 1 h at room temperature washed again four times then imaged.Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

Observed bands - 160 145 and 138 kDa

All lanes:

Western blot - Anti-Peregrin/BRPF1 antibody - N-terminal (<a href='/en-us/products/primary-antibodies/peregrin-brpf1-antibody-n-terminal-ab226909'>ab226909</a>) at 1/500 dilution

Lane 1:

Wild-type HEK-293T cell lysate at 20 µg

Lane 2:

BRPF1 knockout HEK-293T cell lysate at 20 µg

Lane 2:

Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell lysate (ab258795)

Lane 2:

Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-brpf1-peregrin-knockout-hek-293t-cell-line-ab266549'>ab266549</a>)

Lane 3:

PC-3 cell lysate at 20 µg

Lane 4:

HepG2 cell lysate at 20 µg

Predicted band size: 137 kDa

false

Sanger Sequencing - Human BRPF1 (Peregrin) knockout HEK-293T cell lysate (AB258795)
  • Sanger seq

Unknown

Sanger Sequencing - Human BRPF1 (Peregrin) knockout HEK-293T cell lysate (AB258795)

Homozygous : 34 bp deletion in exon 2

Key facts

Cell type

HEK-293T

Species or organism

Human

Tissue

Kidney

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 34 bp deletion in exon 2.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Sanger seq": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab258795-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human BRPF1 knockout HEK293T cell lysate", "number":"AB258795-CMP01", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HEK293T cell lysate", "number":"AB258795-CMP02", "productcode":"" } ] } } }
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Properties and storage information

Gene name
BRPF1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Peregrin/BRPF1 also known as Peregrins is a protein with a molecular mass around 130 kDa. It functions primarily as a component of the MOZ/MORF histone acetyltransferase (HAT) complex. Peregrin/BRPF1 plays a mechanical role in the regulation of gene expression by altering chromatin structure through histone modification. It is expressed in various tissues with higher expression in the brain and testis indicating its importance in neurological and reproductive functions.
Biological function summary

Peregrin/BRPF1 is a part of the MOZ/MORF complex which is important for acetylating histone H3 at lysine 23 influencing transcriptional activation. This action aids in transcription regulation and chromatin remodeling facilitating access of transcription factors to DNA. By participating in these processes Peregrin/BRPF1 helps maintain proper gene expression patterns essential for normal cell function and development.

Pathways

BRPF1 has roles in processes like the cell cycle and embryonic development pathways because its histone acetylation influences gene expression. It interacts with other acetyltransferases such as EP300 and CREBBP which play key roles in transcription regulation and chromatin remodeling within these pathways. These interactions highlight BRPF1's critical involvement in coordinating cellular events and developmental processes.

Dysregulation of BRPF1 is associated with neurological disorders such as intellectual disability and specific cancers due to its role in chromatin remodeling and gene expression. In the context of intellectual disability it interacts with other proteins involved in chromatin structure like NSD1 and KDM6A. These connections illustrate the significant impact of Peregrin/BRPF1's regulation and its influence on health when its function becomes impaired.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Complementary Products

Primary Antibodies

AB259840

Anti-Peregrin/BRPF1 antibody [EPR24069-57]

20ul selling size|RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Peregrin/BRPF1 antibody [EPR24069-57] (AB259840)

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Proteins & Peptides

AB196416

Recombinant Human Peregrin/BRPF1 protein

SDS-PAGE - Recombinant Human Peregrin/BRPF1 protein (AB196416)

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Cell Lines & Lysates

AB266549

Human BRPF1 (Peregrin) knockout HEK-293T cell line

Advanced Validation

Western blot - Human BRPF1 (Peregrin) knockout HEK-293T cell line (AB266549)

  • 0
  • 0

Product promise

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