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AB275500

Human BSG (CD147) knockout A549 cell lysate

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BSG KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 78 bp insertion in exon 5 introducing premature STOP codon.

View Alternative Names

5A11 antigen, 5F7, BASI_HUMAN, Basigin, Basigin (Ok blood group), Blood brain barrier HT7 antigen, Bsg, CD 147, CD147 antigen, Collagenase stimulatory factor, EMMPRIN, Extracellular matrix metalloproteinase inducer, Leukocyte activation antigen M6, M 6, M6 leukocyte activation antigen, Neurothelin, OK, OK blood group, OK blood group antigen, TCSF, Tumor cell-derived collagenase stimulatory factor

5 Images
Western blot - Human BSG (CD147) knockout A549 cell lysate (AB275500)
  • WB

Lab

Western blot - Human BSG (CD147) knockout A549 cell lysate (AB275500)

Lane 1 : Wild-type A549 cell lysate 30 ug
Lane 2 : BSG knockout A549 cell lysate 30 ug
Lane 3 : Raji cell lysate 30 ug
Lane 4 : Jurkat cell lysate 30 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab230921 observed at 42-70 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab230921 was shown to react with CD147 in wild-type A549 cells in western blot with loss of signal observed in BSG knockout cell line ab273748 (knockout cell lysate ab275500). Wild-type and BSG knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab230921 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 500 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD147 antibody [10E10] (<a href='/en-us/products/primary-antibodies/cd147-antibody-10e10-ab230921'>ab230921</a>) at 1/500 dilution

Lane 1:

Wild-type A549 cell lysate at 30 µg

Lane 2:

BSG knockout A549 cell lysate at 30 µg

Lane 2:

Western blot - Human BSG (CD147) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-bsg-cd147-knockout-a549-cell-line-ab273748'>ab273748</a>)

Lane 3:

Raji cell lysate at 30 µg

Lane 4:

Jurkat cell lysate at 30 µg

Predicted band size: 42 kDa

Observed band size: 42-70 kDa

false

Western blot - Human BSG (CD147) knockout A549 cell lysate (AB275500)
  • WB

Lab

Western blot - Human BSG (CD147) knockout A549 cell lysate (AB275500)

Lane 1 : Wild-type A549 cell lysate 30 ug
Lane 2 : BSG knockout A549 cell lysate 30 ug
Lane 3 : Raji cell lysate 30 ug
Lane 4 : Jurkat cell lysate 30 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab119020 observed at 55-70 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab119020 was shown to react with CD147 in wild-type A549 cells in western blot with loss of signal observed in BSG knockout cell line ab273748 (knockout cell lysate ab275500). Wild-type and BSG knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab119020 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at a 1 in 2000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD147 antibody [OTI9B10] (<a href='/en-us/products/primary-antibodies/cd147-antibody-oti9b10-ab119020'>ab119020</a>) at 1/2000 dilution

Lane 1:

Wild-type A549 cell lysate at 30 µg

Lane 2:

BSG knockout A549 cell lysate at 30 µg

Lane 2:

Western blot - Human BSG (CD147) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-bsg-cd147-knockout-a549-cell-line-ab273748'>ab273748</a>)

Lane 3:

Raji cell lysate at 30 µg

Lane 4:

Jurkat cell lysate at 30 µg

Predicted band size: 42 kDa

Observed band size: 55-70 kDa

false

Western blot - Human BSG (CD147) knockout A549 cell lysate (AB275500)
  • WB

Lab

Western blot - Human BSG (CD147) knockout A549 cell lysate (AB275500)

Lane 1 : Wild-type A549 cell lysate 30 ug
Lane 2 : BSG knockout A549 cell lysate 30 ug
Lane 3 : Raji cell lysate 30 ug
Lanes 1 - 3 : Merged signal (red and green). Green - ab108308 observed at 42-70 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.
ab108308 was shown to react with CD147 in wild-type A549 cells in western blot with loss of signal observed in BSG knockout cell line ab273748 (knockout cell lysate ab275500). Wild-type and BSG knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab108308 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD147 antibody [EPR4053] (<a href='/en-us/products/primary-antibodies/cd147-antibody-epr4053-ab108308'>ab108308</a>) at 1/1000 dilution

Lane 1:

Wild-type A549 cell lysate at 30 µg

Lane 2:

BSG knockout A549 cell lysate at 30 µg

Lane 2:

Western blot - Human BSG (CD147) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-bsg-cd147-knockout-a549-cell-line-ab273748'>ab273748</a>)

Lane 3:

Raji cell lysate at 30 µg

Predicted band size: 42 kDa

Observed band size: 42-70 kDa

false

Western blot - Human BSG (CD147) knockout A549 cell lysate (AB275500)
  • WB

Lab

Western blot - Human BSG (CD147) knockout A549 cell lysate (AB275500)

Lane 1 : Wild-type A549 cell lysate 30 ug
Lane 2 : BSG knockout A549 cell lysate 30 ug
Lane 3 : Raji cell lysate 30 ug
Lane 4 : Jurkat cell lysate 30 ug
Lanes 1 - 4 : Merged signal (red and green). Green - ab666 observed at 55-70 kDa. Red - loading control ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) observed at 55kDa.
ab666 was shown to react with CD147 in wild-type A549 cells in western blot with loss of signal observed in BSG knockout cell line ab273748 (knockout cell lysate ab275500). Wild-type and BSG knockout A549 cell lysates were subjected to SDS-PAGE. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with ab666 and ab52866 (Rabbit anti-alpha Tubulin antibody [EP1332Y]) overnight at 4°C at 1 ug/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-CD147 antibody [MEM-M6/1] (<a href='/en-us/products/primary-antibodies/cd147-antibody-mem-m6-1-ab666'>ab666</a>) at 1 µg/mL

Lane 1:

Wild-type A549 cell lysate at 30 µg

Lane 2:

BSG knockout A549 cell lysate at 30 µg

Lane 2:

Western blot - Human BSG (CD147) knockout A549 cell line (<a href='/en-us/products/cell-lines/human-bsg-cd147-knockout-a549-cell-line-ab273748'>ab273748</a>)

Lane 3:

Raji cell lysate at 30 µg

Lane 4:

Jurkat cell lysate at 30 µg

Predicted band size: 42 kDa

Observed band size: 55-70 kDa

false

Sanger Sequencing - Human BSG (CD147) knockout A549 cell lysate (AB275500)
  • Sanger seq

Lab

Sanger Sequencing - Human BSG (CD147) knockout A549 cell lysate (AB275500)

Allele-1 : 78 bp insertion in exon 5 introducing premature STOP codon.

Key facts

Cell type

A549

Species or organism

Human

Tissue

Lung

Knockout validation

Sanger Sequencing,Western blot

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 78 bp insertion in exon 5 introducing premature STOP codon

Disease

Carcinoma

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Reactivity data

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
BSG
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing, Western blot
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CD147 also referred to as EMMPRIN or basigin is a transmembrane glycoprotein with a molecular weight of roughly 50–60 kDa. This protein is found on the surface of many cell types including leukocytes platelets and endothelial cells and it plays a critical role in cell function related to immune responses and cellular interactions. CD147 interacts with several cellular components serving mechanical functions such as facilitating cell-to-cell communication and contributing to the stability of cell structures. The protein is ubiquitously expressed but exhibits higher levels in tissues like the brain skin and liver.
Biological function summary

CD147 engages in multiple roles beyond its mechanical functions. It functions as a part of protein complexes and is involved in processes such as regulation of matrix metalloproteinases (MMPs) which are pivotal in tissue remodeling and repair. It can modulate inflammatory responses and is essential for cellular signaling pathways that affect cellular metabolism and growth. The involvement of CD147 in immune responses indicates its importance in various physiological processes.

Pathways

CD147 plays a significant role in the MAPK and NF-kB signaling pathways which are fundamental for controlling inflammatory responses and cell survival. It interacts with proteins such as integrins and cyclophilins which are important for mediating these pathways. These interactions highlight CD147's impact on cellular dynamics and its potential role in modulating the cellular environment in response to external stimuli.

CD147 has been implicated in cancer promotion and progression as well as inflammatory diseases like rheumatoid arthritis. Its overexpression is often observed in tumors where it interacts with MMPs to facilitate tumor invasion and metastasis. In inflammatory conditions CD147 can influence the behavior of proteins like cytokines exacerbating symptoms and contributing to disease severity. These associations make CD147 a potential target for therapeutic intervention in cancer and inflammatory diseases.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 1 US: 1

Adherent/suspension

Adherent

Gender

Male

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Product protocols

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Complementary Products

Primary Antibodies

AB108308

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BOND RX™ Validated|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD147 antibody [EPR4053] (AB108308)

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Assay Kits

AB219631

Human EMMPRIN ELISA Kit

KO Validated|Recombinant|SimpleStep

Sandwich ELISA - Human EMMPRIN ELISA Kit (AB219631)

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Proteins & Peptides

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Proteins & Peptides

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Product promise

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