Human BTRC (Beta TRCP) knockout HEK-293T cell lysate
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BTRC KO cell lysate available now. KO validated by Western blot. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon5 and 4 bp deletion in exon5.
View Alternative Names
pIkappaBalpha-E3 receptor subunit, E3RSIkappaB, F-box/WD repeat-containing protein 1A, BTRC, Beta TRCP/HOS, F-box and WD repeats protein beta-TrCP, BTRCP, FBXW1A, Epididymis tissue protein Li 2a, FBW1A
- WB
Lab
Western blot - Human BTRC (Beta TRCP) knockout HEK-293T cell lysate (AB257864)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : HEK-293T cell lysate 20 μg
Lane 3 : HepG2 cell lysate 20 μg
Lane 4 : PANC-1 cell lysate 20 μg
False colour image of Western blot : Anti-BTRC antibody staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to BTRC. A band was observed at 60/63/67 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BTRC knockout cell line ab266201 (knockout cell lysate ab257864). To generate this image, wild-type and BTRC knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Anti-BTRC antibody at 1/500 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human BTRC (Beta TRCP) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-btrc-beta-trcp-knockout-hek-293t-cell-line-ab266201'>ab266201</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
PANC-1 cell lysate at 20 µg
false
- WB
Lab
Western blot - Human BTRC (Beta TRCP) knockout HEK-293T cell lysate (AB257864)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : HEK-293T cell lysate 20 μg
Lane 3 : HepG2 cell lysate 20 μg
Lane 4 : PANC-1 cell lysate 20 μg
False colour image of Western blot : Anti-BTRC antibody staining at 1/500 dilution, shown in black; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to BTRC. A band was observed at 60/63/67 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BTRC knockout cell line ab266201 (knockout cell lysate ab257864). To generate this image, wild-type and BTRC knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % BSA in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times before development with Optiblot (ECL reagent ab133456) and imaged with 20 minutes exposure time. Secondary antibodies used were HRP conjugated Goat anti-Rabbit (H+L) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Anti-BTRC antibody at 1/500 dilution
Lane 1:
Wild-type HEK-293T cell lysate# at 20 µg
Lane 2:
HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human BTRC (Beta TRCP) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-btrc-beta-trcp-knockout-hek-293t-cell-line-ab266201'>ab266201</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
PANC-1 cell lysate at 20 µg
false
- WB
Lab
Western blot - Human BTRC (Beta TRCP) knockout HEK-293T cell lysate (AB257864)
Lane 1 : Wild-type HEK-293T cell lysate 20 μg
Lane 2 : HEK-293T cell lysate 20 μg
Lane 3 : HepG2 cell lysate 20 μg
Lane 4 : PANC-1 cell lysate 20 μg
False colour image of Western blot : Anti-BTRC antibody staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, the antibody was shown to bind specifically to BTRC. A band was observed at 60/63/67 kDa in wild-type HEK-293T cell lysates with no signal observed at this size in BTRC knockout cell line ab266201 (knockout cell lysate ab257864). To generate this image, wild-type and BTRC knockout HEK-293T cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
All lanes:
Anti-BTRC antibody at 1/500 dilution
Lane 1:
Wild-type HEK-293T cell lysate at 20 µg
Lane 2:
HEK-293T cell lysate at 20 µg
Lane 2:
Western blot - Human BTRC (Beta TRCP) knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-btrc-beta-trcp-knockout-hek-293t-cell-line-ab266201'>ab266201</a>)
Lane 3:
HepG2 cell lysate at 20 µg
Lane 4:
PANC-1 cell lysate at 20 µg
false
- Sanger seq
Unknown
Sanger Sequencing - Human BTRC (Beta TRCP) knockout HEK-293T cell lysate (AB257864)
Allele-2 : 1 bp insertion in exon5
- Sanger seq
Unknown
Sanger Sequencing - Human BTRC (Beta TRCP) knockout HEK-293T cell lysate (AB257864)
Allele-1 : 4 bp deletion in exon5
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Reactivity data
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Beta TRCP participates in essential functions by being a component of the SCF (Skp1-Cullin-F-box) complex which facilitates the ubiquitination of its substrates. SCF complexes regulate various cellular processes by targeting proteins that need timely degradation. As a part of the complex beta TRCP controls the stability of pivotal substrates ensuring proper regulation of biological events like DNA replication and repair.
Pathways
Beta TRCP plays critical roles in the Wnt and NF-kB signaling pathways. These pathways depend on the precise regulation of protein levels allowing for proper cellular responses. Beta TRCP interacts with key proteins such as IkB in the NF-kB pathway and beta-catenin in the Wnt pathway controlling their ubiquitination and stability. The degradation of these proteins is necessary for pathway activation or repression influencing cell proliferation and survival decisions.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com