Human BZW1 (Protein Orf) knockout HEK-293T cell lysate
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BZW1 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon3 and Insertion of the selection cassette in exon3.
View Alternative Names
BZAP45, BZW1_HUMAN, Basic leucine zipper and W2 domain-containing protein 1, KIAA0005, Nbla10236, Protein Orf, basic leucine-zipper protein BZAP45, putative protein product of Nbla10236
- Sanger seq
Unknown
Sanger Sequencing - Human BZW1 (Protein Orf) knockout HEK-293T cell lysate (AB263116)
Allele-1 : Insertion of the selection cassette in exon3
- Sanger seq
Unknown
Sanger Sequencing - Human BZW1 (Protein Orf) knockout HEK-293T cell lysate (AB263116)
Allele-2 : 1 bp insertion in exon3
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
BZW1 influences cell growth and proliferation by engaging in the regulation of gene transcription. It can interact with other proteins to form complexes that modulate these processes. BZW1 adjusts protein synthesis rates by acting at the level of translational control affecting cellular responses to different growth conditions. Its function supports overall cellular homeostasis by ensuring proteins are produced as needed.
Pathways
BZW1 is integral to both the mTOR and eIF2 signaling pathways which are critical in regulating protein synthesis and stress responses. It is related to other proteins like mTOR and eIF2α within these pathways and its activity can influence how cells respond to nutrient availability and environmental changes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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