Human C11orf31 knockout HEK-293T cell lysate
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SELENOH KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 2 bp insertion in exon 1.
View Alternative Names
C11orf31, Chromosome 11 open reading frame 31, SELH_HUMAN, Selenoprotein H
- Sanger seq
Unknown
Sanger Sequencing - Human C11orf31 knockout HEK-293T cell lysate (AB263117)
Homozygous : 2 bp insertion in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Zygosity
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
Selenoprotein H contributes to protecting cells from oxidative damage. It plays a role in regenerating antioxidant molecules and maintaining cellular redox balance. SELH is not known to be part of a larger protein complex but works in concert with other antioxidant molecules to enhance cellular resistance to oxidative stress. It also involves gene regulation particularly through modulating transcription factors that respond to oxidative signals.
Pathways
Selenoprotein H engages significantly in the cellular antioxidant pathways and redox homeostasis. It interacts with enzymes like glutathione peroxidase and thioredoxin reductases which are essential in countering oxidative stress. These interactions help maintain signal transduction pathways which depend on redox-sensitive components affecting cellular metabolism and survival. SELH along with these proteins ensures proper cellular function by modulating oxidative status and impacting pathways related to energy metabolism and apoptosis.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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