Human C12orf57 (GRCC10) knockout HEK-293T cell lysate
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- Sanger seq
Unknown
Sanger Sequencing - Human C12orf57 (GRCC10) knockout HEK-293T cell lysate (AB258798)
Allele-1 : 2 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human C12orf57 (GRCC10) knockout HEK-293T cell lysate (AB258798)
Allele-2 : Insertion of the selection cassette in exon 1
Complementary Products
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
GRCC10 plays a significant role in cell communication and signaling. It functions as part of a larger receptor complex which allows it to effectively transmit signals from the extracellular environment to the cell's interior. This signaling affects numerous biological processes such as cell proliferation differentiation and apoptosis. GRCC10's activity is important for maintaining the balance between these cellular outcomes which is important for normal development and function.
Pathways
GRCC10 participates in important signaling networks such as the MAPK/ERK and PI3K/AKT pathways. These pathways control cell growth survival and metabolism. GRCC10 interacts with proteins like RAF and AKT within these pathways helping to modulate their activity. Through its pathway involvement GRCC10 contributes to the regulation of important biological processes including response to hormones and growth factors.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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