Human C17orf89 knockout HEK-293T cell lysate
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NDUFAF8 KO cell lysate available now. KO validated by. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 10 bp deletion in exon 1 and 2 bp insertion in exon 1 and Insertion of the selection cassette in exon 1.
View Alternative Names
Chromosome 17 Open Reading Frame 89, Uncharacterized Protein C17orf89
- Sanger seq
Unknown
Sanger Sequencing - Human C17orf89 knockout HEK-293T cell lysate (AB263121)
Allele-2 : 2 bp insertion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human C17orf89 knockout HEK-293T cell lysate (AB263121)
Allele-1 : 10 bp deletion in exon 1
- Sanger seq
Unknown
Sanger Sequencing - Human C17orf89 knockout HEK-293T cell lysate (AB263121)
Allele-3 : Insertion of the selection cassette in exon 1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C17orf89 has roles involving cellular structural integrity and signaling. It does not belong to any known protein complex but it may still coordinate actions within the cell membrane. Through these functions C17orf89 helps maintain an optimal cellular environment supporting the function of cells and tissues. Although its precise biological functions remain studied its interactions indicate involvement in critical cellular maintenance tasks.
Pathways
The protein interacts with elements of the insulin signaling pathway and possibly the mTOR pathway influencing metabolic processes. In these pathways C17orf89 interacts with proteins like insulin receptor substrate 1 (IRS1) and the mammalian target of rapamycin (mTOR). These interactions suggest a role in nutrient sensing and cellular growth responses enabling proper cellular adaptation to environmental changes.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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