Human C1orf131 knockout HeLa cell lysate
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FSAF1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp deletion in exon1 and 1 bp insertion in exon1.
View Alternative Names
C1orf131, CA131_HUMAN, Chromosome 1 open reading frame 131, DKFZp547B1713, Hypothetical protein LOC128061, OTTHUMP00000036145, OTTHUMP00000036146, RP4-609B14.1, Uncharacterized protein C1orf131
- Sanger seq
Unknown
Sanger Sequencing - Human C1orf131 knockout HeLa cell lysate (AB263127)
Allele-2 : 1 bp insertion in exon1
- Sanger seq
Unknown
Sanger Sequencing - Human C1orf131 knockout HeLa cell lysate (AB263127)
Allele-1 : 1 bp deletion in exon1
Product details
Knockout cell lysate achieved by CRISPR/Cas9.
REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.
User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.
This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.
What's included?
Properties and storage information
Gene name
Gene editing type
Gene editing method
Knockout validation
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
C1orf131 serves a functional role linked to cellular processes and regulatory mechanisms. It might belong to a complex that regulates intracellular signaling pathways although specific interactions remain to be fully clarified. Its broad expression suggests functions in basic cellular activities possibly affecting cell growth and communication. As scientists uncover more understanding the exact biological roles of C1orf131 continues to expand.
Pathways
C1orf131 may integrate into broad signaling networks that control cellular proliferation and maintenance. One potential interaction is with the PI3K/Akt pathway an important regulatory pathway for cell survival and metabolism. Within this pathway C1orf131 could have associative functions connecting with regulatory proteins like the AKT1. Connections to these pathways suggest C1orf131’s involvement in cellular homeostasis and adaptation.
Quality control
STR analysis
CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX
Cell culture
Biosafety level
EU: 2 US: 2
Adherent/suspension
Adherent
Gender
Female
Complementary Products
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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