JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB263129

Human C5orf51 knockout HeLa cell lysate

Be the first to review this product! Submit a review

|

(0 Publication)

RIMOC1 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.
1 Images
Sanger Sequencing - Human C5orf51 knockout HeLa cell lysate (AB263129)
  • Sanger seq

Unknown

Sanger Sequencing - Human C5orf51 knockout HeLa cell lysate (AB263129)

Homozygous : 1 bp insertion in exon1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, 1 bp insertion in exon1.

Disease

Adenocarcinoma

style
left-column

Complementary Products

Cell Lines & Lysates

AB265321

Human C5orf51 knockout HeLa cell line

Sanger Sequencing - Human C5orf51 knockout HeLa cell line (AB265321)

  • 0
  • 0
style
left-column

Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute, ERS Genomics Limited and Sigma-Aldrich Co. LLC, and is developed with patented technology. For full details of the licenses and patents please refer to our limited use license and patent pages.

style
left-column

What's included?

{ "values": { "1Kit": { "sellingSize": "1 Kit", "publicAssetCode":"ab263129-1Kit", "assetComponentDetails": [ { "size":"1 x 100 µg", "name":"Human C5orf51 knockout HeLa cell lysate", "number":"AB263129-CMP02", "productcode":"" }, { "size":"1 x 100 µg", "name":"Human wild-type HeLa cell lysate", "number":"AB263129-CMP01", "productcode":"" } ] } } }
style
left-column

Properties and storage information

Gene name
RIMOC1
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C5orf51 also known as Chromosome 5 open reading frame 51 is a lesser-known protein that has a molecular weight of approximately 32 kDa. This protein is mainly expressed in the testis but lower levels of expression can be found in other tissues. It is a non-enzymatic protein indicating its role might not involve catalytic activity but possibly structural or regulatory functions. Because of its expression pattern it has been minimally characterized yet scientists consider it an area open for further exploration.
Biological function summary

C5orf51 plays a role that is not yet fully understood but researchers suspect it might be involved in regulating cellular processes in the tissues it is expressed in. While it is not currently identified as part of a larger protein complex its localization hints at potential interactions within the cellular environment. Much work remains to determine its exact biological functions and interactions.

Pathways

Current research suggests that C5orf51 could intersect with pathways involved in reproductive or germ cell function due to its primary expression in the testis. Scientists postulate potential interactions with proteins involved in spermatogenesis though these associations are largely speculative. Pathway analysis continues to be an important step in uncovering the broader role of this protein.

Scientists have yet to establish direct links between C5orf51 and specific diseases. However its expression profile implies a potential connection to reproductive health such as infertility. Researchers continue investigating if C5orf51 interacts with proteins implicated in testis-related disorders searching for evidence that may illuminate its impact on human health. As studies progress additional associations with other conditions could emerge.
style
left-column

Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

style
left-column

Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

style
left-column

Product protocols

style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com