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AB263130

Human C6orf62 knockout HeLa cell lysate

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C6orf62 KO cell lysate available now. KO validated. Free of charge wild type control included. Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.
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Sanger Sequencing - Human C6orf62 knockout HeLa cell lysate (AB263130)
  • Sanger seq

Unknown

Sanger Sequencing - Human C6orf62 knockout HeLa cell lysate (AB263130)

Homozygous : 1 bp deletion in exon 1

Key facts

Cell type

HeLa

Species or organism

Human

Tissue

Cervix

Knockout validation

Sanger Sequencing

Mutation description

Knockout achieved by using CRISPR/Cas9, Homozygous: 1 bp deletion in exon 1.

Disease

Adenocarcinoma

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Complementary Products

Cell Lines & Lysates

AB265397

Human C6orf62 knockout HeLa cell line

Sanger Sequencing - Human C6orf62 knockout HeLa cell line (AB265397)

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Product details

Knockout cell lysate achieved by CRISPR/Cas9.

REACH authorisation
Abcam has not and does not intend to apply for the REACH Authorisation of customers' uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

Lysate preparation: Our lysates are made using RIPA buffer to which we add a protease inhibitor cocktail and phosphatase inhibitor cocktail (ratio: 300:100:10). This means that the protein of interest is denatured. If you require a native form of the protein please use the live cell version. Please refer to our lysis protocol for further details on how our lysates are prepared.

User storage instructions: Lyophilizate may be stored at 4°C. After reconstitution, store at -20°C for short-term storage or -80°C for long-term storage.

This product is subject to limited use licenses from The Broad Institute and ERS Genomics Limited, and is developed with patented technology. For full details of the limited use licenses and relevant patents please refer to our limited use license and patent pages.

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What's included?

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Properties and storage information

Gene name
C6orf62
Gene editing type
Knockout
Gene editing method
CRISPR technology
Knockout validation
Sanger Sequencing
Zygosity
Homozygous
Shipped at conditions
Ambient - Can Ship with Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C6orf62 also known as Chromosome 6 Open Reading Frame 62 is a protein of roughly 36 kDa. This protein plays a mechanical role related to cell cycle regulation and is expressed mainly in tissues like liver and kidney. It does not act alone; instead it often interacts with other cellular components influencing cellular processes through its interactions.
Biological function summary

The function of this protein involves regulation of cell proliferation and apoptosis. C6orf62 is not a single-acting entity but is thought to be part of a larger protein complex. This association allows it to maintain normal cellular functions by modulating expression levels of genes important for growth and programmed cell death.

Pathways

C6orf62 has key roles in the cell cycle and apoptotic pathways. It associates with proteins like cyclins and caspases. Cyclins are involved in progression through different phases of the cell cycle while caspases are important for the initiation of apoptosis. C6orf62 helps balance these pathways depending on the cellular context ensuring proper function and survival of the cell.

Studies suggest connections of C6orf62 with cancers and neurodegenerative disorders. Dysregulation of this protein may affect its associated pathways leading to uncontrolled cell growth as seen in certain cancers or improper cell death in neurodegenerative diseases. Through its connections in these pathways C6orf62 also potentially interacts with oncogenes and neuroprotective proteins impacting disease progression and pathology.
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Quality control

STR analysis

CSF1PO, D13S317, D7S820, D5S818, TH01, D16S539, TPOX

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Cell culture

Biosafety level

EU: 2 US: 2

Adherent/suspension

Adherent

Gender

Female

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Product protocols

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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